中国中药杂志

2021, v.46(02) 391-397

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连续单因素法优化黄芪中黄芪甲苷的含量测定方法
Optimization of determination of astragaloside Ⅳ in Astragali Radix by continuous single-factor method

梁曜华;李春;冯伟红;刘晓谦;陈两绵;朱晶晶;闫利华;孟辰笑凝;郭中原;高慧敏;王智民;
LIANG Yao-hua;LI Chun;FENG Wei-hong;LIU Xiao-qian;CHEN Liang-mian;ZHU Jing-jing;YAN Li-hua;MENG Chen-xiao-ning;GUO Zhong-yuan;GAO Hui-ming;WANG Zhi-min;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines;Key Laboratory of SATCM for Process Analysis Technology of Chinese Herbal Medicines;

摘要(Abstract):

针对法定标准黄芪项下黄芪甲苷测定中供试液制备方法繁琐、不稳定,黄芪甲苷提取不充分的缺陷,该文采用HPLC-ELSD对影响黄芪甲苷含量的7个主要因素进行连续单因素分析优化了其供试液制备工艺。该方法精密度、重复性、稳定性试验结果均良好,黄芪甲苷的平均加样回收率为99.65%,RSD为2.2%。黄芪甲苷的进样量在0.46~9.1μg,其对数与峰面积对数成良好线性关系,r=0.999 6。采用新建立的方法对29批黄芪样品进行了测定,测定结果与法定方法测定结果进行比较,结果发现前者平均含量为后者的1.458倍,说明新的样品处理方法提取黄芪甲苷更充分,而且简单、可靠。依据测定结果,建议新版药典提高黄芪中黄芪甲苷的含量标准。
This paper aims to solve the problems of complicated-unstable test solution preparation process and insufficient extraction of the active ingredient astragaloside Ⅳ in the legal method for the determination of astragaloside Ⅳ in Astragali Radix. The continuous single-factor analysis of seven main factors affecting the content of astragaloside Ⅳ was carried out by HPLC-ELSD, and then the pre-paration method of test solution was optimized. This optimized method exhibited excellent performance in precision, repeatability and stability. The average recovery rate of astragaloside Ⅳ was 99.65% with RSD 2.2%. Astragaloside Ⅳ showed a good linearity between the logarithm of peak area and the logarithm of injection quantity in the range of 0.46-9.1 μg(r=0.999 6). The contents of astragaloside Ⅳ in 29 batches of Astragali Radix were determined by the new and the legal methods. The results showed that the average content of astragaloside Ⅳ in these Astragali Radix samples determined by the former method was 1.458 times than that of the latter one, indicating the new method was simple, reliable and more adequate to extract target compound. According to the results, it is suggested to improve the content standard of astragaloside Ⅳ in Astragali Radix in the new edition of Chinese Pharmacopeia.

关键词(KeyWords): 黄芪甲苷;含量测定;连续单因素法;黄芪;优化
astragaloside Ⅳ;determination;continuous single-factor method;Astragali Radix;optimization

Abstract:

Keywords:

基金项目(Foundation): 国家中药标准化项目(ZYBZH-C-GD-07)

作者(Authors): 梁曜华;李春;冯伟红;刘晓谦;陈两绵;朱晶晶;闫利华;孟辰笑凝;郭中原;高慧敏;王智民;
LIANG Yao-hua;LI Chun;FENG Wei-hong;LIU Xiao-qian;CHEN Liang-mian;ZHU Jing-jing;YAN Li-hua;MENG Chen-xiao-ning;GUO Zhong-yuan;GAO Hui-ming;WANG Zhi-min;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines;Key Laboratory of SATCM for Process Analysis Technology of Chinese Herbal Medicines;

DOI: 10.19540/j.cnki.cjcmm.20200706.203

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