中国中药杂志

2007, (24) 2597-2600

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RP-HPLC快速测定清胃黄连片中4种指标成分的含量
Fast and simultaneous determination of four index components in Qingwei Huanglian tablets by RP-HPLC

李艳荣;蒋晔;郝福;丁翔宇;
LI Yan-rong,JIANG Ye,HAO Fu,DING Xiang-yu(School of Pharmacy,Hebei Medical University,Shijiazhuang 050017,China)

摘要(Abstract):

目的:建立RP-HPLC测定清胃黄连片中栀子苷、黄芩苷、盐酸小檗碱和甘草酸铵4种指标成分含量的方法。方法:采用Hypersil C18色谱柱(4.6 mm×250 mm,5.0μm);流动相为乙腈-0.5%三乙胺水溶液(磷酸调pH3.0)采用梯度洗脱,检测波长230 nm;流速1.0 mL.min-1。结果:栀子苷、黄芩苷、盐酸小檗碱和甘草酸铵的线性范围分别为:4.82~77.2,5.80~92.8,1.63~26.1,6.40~102μg.mL-1(0.999 7Objective: To establish a RP-HPLC method for fast and simultaneous determination of four index contents,which are Genpioside,Baicalin,Berberine Hydrochloride and Ammonium Glyeyrrhizinate,in Qingwei Huanglian tablets.Method: A separation was well achieved by gradient elution on a Hypersil C18(4.6 mm×250 mm,5 μm) column with the mobile phases of acetontrile-0.5 % triethylamine water-solution(pH 3.0) at a flow rate of 1.0 mL·min-1,detection wavelength of 230 nm,and room temperature.Result: The calibration curve were linear in the ranges of 4.82-77.2,5.80-92.8,1.63-26.1 and 6.40-102 μg·mL-1(r=0.999 7-0.999 9) for genpioside,baicalin,berberine hydrochloride and ammonium glyeyrrhizinate,respectively.The average recoveries of four index components were not less than 98.0 %.Conclusion: The method is convenient,rapid and accurate.It is suitable for the quality control of Qingwei Huanglian tablets.

关键词(KeyWords): RP-HPLC;清胃黄连片;栀子苷;黄芩苷;盐酸小檗碱;甘草酸铵
RP-HPLC;Qingwei Huanglian tablets;genpioside;baicalin;berberine hydrochloride;ammonium glyeyrrhizinate

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基金项目(Foundation): 河北省科技公关计划项目(062761956)

作者(Author): 李艳荣;蒋晔;郝福;丁翔宇;
LI Yan-rong,JIANG Ye,HAO Fu,DING Xiang-yu(School of Pharmacy,Hebei Medical University,Shijiazhuang 050017,China)

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