中国中药杂志

2016, v.41(19) 3597-3601

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

5-氮杂胞苷影响LjFNS Ⅱ1.1和LjFNS Ⅱ2.1催化金银花木犀草素和木犀草苷合成机制的研究
Research of regulating synthesis of luteolin and luteoloside of Lonicera japonica by Lj FNS Ⅱ1.1 and Lj FNS Ⅱ2.1 treated with 5-aza C

刘爽;杨健;查良平;赵玉洋;刘勇;袁媛;黄璐琦;
LIU Shuang;YANG Jian;ZHA Liang-ping;ZHAO Yu-yang;LIU Yong;YUAN Yuan;HUANG Lu-qi;School of Chinese Pharmacy,Beijing University of Chinese Medicine;State Key Laboratory Breeding Base of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;

摘要(Abstract):

该研究通过对金银花叶片进行不同时间(1,2,3 d)与不同浓度(20,40,60,80,100μmol·L-1)的5-氮杂胞苷处理,探索5-氮杂胞苷影响黄酮合酶FNSⅡ催化木犀草素和木犀草苷合成的机制。首先对Lj FNSⅡ1.1,Lj FNSⅡ2.1进行克隆;其次UPLC-MS/MS测定金银花叶中木犀草素与木犀草苷的含量,以及Real-Time PCR分析Lj FNSⅡ1.1,Lj FNSⅡ2.1的表达水平,结果表明Lj FNSⅡ1.1,Lj FNSⅡ2.1基因的表达水平与木犀草素的含量变化趋势大体一致,但与木犀草苷的含量变化相关性不显著;同时发现二者之间的表达水平略有差异。研究结果表明,5-氮杂胞苷处理金银花叶片后,Lj FNSⅡ1.1,Lj FNSⅡ2.1的表达水平上升,从而调控木犀草素和木犀草苷的含量升高,为研究Lj FNSⅡ1.1,Lj FNSⅡ2.1催化木犀草素和木犀草苷合成机制提供技术支撑和理论基础。
This study is aimed to explore the mechanism of catalyzing the synthesis of luteolin and luteoloside by Lj FNS Ⅱ 1. 1 and Lj FNS Ⅱ 2. 1. The leaves of Lonicera japonica were treated with different concentrations of 5-aza C( 20,40,60,80,100 μmol·L-1) for three periods( 1,2,3 d). Firstly,we cloned Lj FNS Ⅱ 1. 1 and Lj FNS Ⅱ 2. 1. Secondly,we analyzed the expression levels of Lj FNSⅡ 1. 1 and Lj FNS Ⅱ 2. 1 by Real-Time PCR and the contents of luteolin and luteoloside determined by UPLC-MS/MS. The results explained the expression levels of Lj FNS Ⅱ 1. 1 and Lj FNS Ⅱ 2. 1 consistent with the content variation of luteolin in general,but there was no significant correlation with the contents of luteoloside. And we found the expression levels of Lj FNS Ⅱ 1. 1 and Lj FNS Ⅱ 2. 1were slightly different. The research indicated that the contents of luteolin and luteoloside got higher by improving the expression levels of Lj FNS Ⅱ 1. 1 and Lj FNS Ⅱ 2. 1. This will provide technical support and lay a theoretical foundation for regulating the synthesis of luteolin and luteoloside by Lj FNS Ⅱ 1. 1 and Lj FNS Ⅱ 2. 1.

关键词(KeyWords): 金银花;黄酮合酶Ⅱ;5-氮杂胞苷;木犀草素;木犀草苷
Lonicera japonica;flavone synthases Ⅱ;5-aza C;luteolin;luteoloside

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81373959)

作者(Author): 刘爽;杨健;查良平;赵玉洋;刘勇;袁媛;黄璐琦;
LIU Shuang;YANG Jian;ZHA Liang-ping;ZHAO Yu-yang;LIU Yong;YUAN Yuan;HUANG Lu-qi;School of Chinese Pharmacy,Beijing University of Chinese Medicine;State Key Laboratory Breeding Base of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;

Email:

DOI:

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享