中国中药杂志

2011, v.36(16) 2207-2210

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蟾衣化学成分及体外抗肿瘤活性研究
Chemical constituents from Bufonis periostracum and their antitumor activity in vitro

高慧敏;吴喜燕;李宗云;游云;张毅;王智民;
GAO Huimin1,2,WU Xiyan1,2,LI Zongyun1,2,YOU Yun1,ZHANG Yi1,WANG Zhimin1,2(1.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China; 2.National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine,Beijing 100700,China)

摘要(Abstract):

目的:对源于蟾蜍的新资源蟾衣进行化学成分研究,并对蟾衣提取物及分离得到的主要化合物进行抗肿瘤活性评价。方法:蟾衣粗粉采用95%乙醇回流提取,提取物采用正相硅胶、反相硅胶和葡聚糖凝胶Sephadex LH-20柱色谱分离,结合结晶法对化合物进行纯化,通过波谱分析鉴定化合物的结构;采用MTT法对95%乙醇提取物及分离得到的主要化合物进行体外抗肿瘤活性评价。结果:从蟾衣95%乙醇提取物中分离鉴定了8个化合物,分别为棕榈酸胆甾烯酯(1),胆甾醇(2),5α,8α-epidioxycholesta-6-en-3β-ol(3),胆甾-5-烯-3β,7β-二醇(4),胆甾-7-烯-3β,5α,6β-三醇(5),3-十八烷氧基-1,2-丙二醇(6),Δ4,5(E),Δ9,10(Z)-鞘胺醇-正十五碳酸酰胺(7)和蟾蜍噻咛(8);抗肿瘤活性筛选表明,蟾衣95%乙醇提取物和分离得到的主要化合物对试验的细胞株均无抑制作用。结论:化合物1~8均为首次从蟾衣中分离得到,其中化合物3,5~7为首次从中华大蟾蜍和蟾蜍属中分离得到。
Eight compounds were isolated from Bufonis periostracum by repeated column chromatography on silica gel,ODS and Sephadex LH-20 and their structures were characterized as palmitatic acid cholesteryl ester(1),cholesterol(2),5α,8α-epidioxycholesta-6-en-3β-ol(3),cholest-5-en-3β,7β-diol(4),cholest-7-en-3β,5α,6β-triol(5),3-octaddecyloxy-1,2-propanediol(6),isisamide(7) and bufothionine(8) on the base of spectral analysis.Compounds 1-8 were isolated from Bufonis periostracum for the first time and compounds 3,5,6,7 were obtained from Bufo bufo gargarizans and Bufo genus for the first time.The bioassays showed all tested samples displayed no antitumor activity against the cell lines such as A549,BeL 7402,HGC-27 and HL-60,except the control compound bufalin.

关键词(KeyWords): 蟾衣;中华大蟾蜍;化学成分;抗肿瘤
Bufonis periostracum;Bufo bufo gargarizans;chemical constituents;antitumor

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(30801512);; 国家“重大新药创制”科技重大专项(2009ZX09301-005,2009ZX09308-003);; 中国中医科学院自选课题(Z02073)

作者(Author): 高慧敏;吴喜燕;李宗云;游云;张毅;王智民;
GAO Huimin1,2,WU Xiyan1,2,LI Zongyun1,2,YOU Yun1,ZHANG Yi1,WANG Zhimin1,2(1.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China; 2.National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine,Beijing 100700,China)

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