中国中药杂志

2018, v.43(11) 2177-2183

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基于“劳则气耗”理论研究气虚证动物模型的建立方法
Study on establishment of an animal model of Qi deficiency syndrome base on theory of over exertion leading to Qi consumption in traditional Chinese medicine

陈进成;刘建勋;林成仁;任钧国;李磊;郭浩;
CHEN Jin-cheng;LIU Jian-xun;LIN Cheng-ren;REN Jun-guo;LI Lei;GUO Hao;Beijing Key Laboratory of Pharmacology of Chinese Materia Medica,Institute of Basic Medical Sciences of Xiyuan Hospital,China Academy of Chinese Medical Sciences;Postdoctoral Research Station,China Academy of Chinese Medical Sciences;

摘要(Abstract):

该研究根据"劳则气耗"理论,旨在通过比较"神劳"与"体劳"2种因素研究气虚证大鼠模型的建立方法与系统评价体系。分别以水环境睡眠剥夺的"神劳"与以负重力竭游泳的"体劳"为干预因素,按"拟临床研究"的原则建立气虚证动物模型,实验分为正常组、力竭游泳组、睡眠剥夺组与复合组(力竭游泳复合睡眠剥夺),力竭游泳采用负重5%进行,每日1次直至力竭;睡眠剥夺采用水环境多平台睡眠剥夺法,每日随机1416 h,复合组则2种方法联合应用,实验周期为6周。采用开场实验观察运动总路程、平均速度、活动时间以及进入中央区域次数,游泳力竭法检测力竭游泳时间,超声心动图检测心功能(LVEF,CO,FS,LVDd),舌象采集分析舌面色彩饱和度,小动物无创脉搏血氧仪测定脉搏幅度,ELISA法检测血清中ATP,ADP含量,血凝仪检测凝血四项。结果显示,睡眠剥夺组自主活动、体质量、运动能力降低明显,神经功能损伤明显,心功能下降,舌面色彩呈现淡白,ATP含量显著降低,ADP显著升高,脉搏幅度显著降低。通过采用"四诊合参"系统评价体系比较"神劳"与"体劳"2种干预因素,以"神劳"为特征的睡眠剥夺方法更适于建立拟临床的气虚证动物模型。
Based on the theory of " overexertion leading to Qi consumption",this study aimed to compare the intervention factors of nervous and physical fatigue to establish a rat model of Qi deficiency syndrome,moreover,the systematic evaluationsystem was necessary with the standard of syndrome diagnosis of Qi deficiency. According to the clinical-mimetic principle the Qi deficiency syndrome animal model is established by the factors of sleep deprivation( SD) and exhaustive swimming( ES). Rats were randomly divided into four groups,normal group,SD group,ES group and compound group( exhaustion swimming complex sleep deprivation). The method of ES was force rats to swim until the exhaustion with 5% of weight,and the SD method by using multi-platform sleep deprivation of water environment randomly 14-16 h daily,and the complex set by using of two methods was combined. The movement distance,average speed,activity time and numbers into the central area were detected by open field test,swimming exhaustion time was detected by swimming exhaustion method,the cardiac function( LVEF,CO,FS,LVDd) was detected by echocardiography,tongue imaging analysis and pulsedistention as the index of tongue and pulse diagnosis,the content of ATP and ADP in serum was detected by ELISA,the blood coagulation indexes for blood stasis syndrome as the additional. These results indicated that independent activity,body weight and exercise capacity were decreased significantly,the neurological function injured significantly,heart function was decreased significantly,the tongue surface color was pale white,and the pulse distention was decreased significantly. The content of ATP decreased significantly and the ADP increased significantly. By the method of four-diagnostic evaluation system to compare the factors of SD and ES,the Qi deficiency syndrome animalmodel could be successfully established by sleep deprivation method.

关键词(KeyWords): 睡眠剥夺;游泳力竭;气虚证;证候模型
sleep deprivation;exhaustive swimming;Qi deficiency syndrome;syndrome model

Abstract:

Keywords:

基金项目(Foundation): 国家重点基础研究发展计划(973)项目(2015CB554405)

作者(Authors): 陈进成;刘建勋;林成仁;任钧国;李磊;郭浩;
CHEN Jin-cheng;LIU Jian-xun;LIN Cheng-ren;REN Jun-guo;LI Lei;GUO Hao;Beijing Key Laboratory of Pharmacology of Chinese Materia Medica,Institute of Basic Medical Sciences of Xiyuan Hospital,China Academy of Chinese Medical Sciences;Postdoctoral Research Station,China Academy of Chinese Medical Sciences;

DOI: 10.19540/j.cnki.cjcmm.20180418.003

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