中国中药杂志

2007, (14) 1449-1452

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大豆苷元对压力负荷性心肌肥厚大鼠心室重构的影响
Influence of daidzein on left ventricular remodeling in rats with hypertrophic myocardium induced by pressure overload

周俐;周青;熊小琴;刘建新;何蔚;
ZHOU Li,ZHOU Qing,XIONG Xiao-qing,LIU Jian-xin,HE Wei(Gannan Medical College,Ganzhou 341000,China)

摘要(Abstract):

目的:研究大豆苷元(daidzein,DD)对压力负荷性心肌肥厚大鼠心室重构的保护作用及其机制。方法:采用腹主动脉缩窄法制备大鼠心肌肥厚模型。分假手术组、模型组、DD(30,60,120 mg·kg-1)治疗组;4周后处死大鼠,测量大鼠全心质量指数(HW/BW)和左室质量指数(LVW/BW即LVI),测定左室心肌纤维直径(MD),检测左室心肌组织羟脯氨酸(Hyp)、一氧化氮(NO)、血管紧张素Ⅱ(AngⅡ)含量和一氧化氮合酶(NOS),Na+-K+-ATPase,Ca2+-ATPase活性;检测血浆AngⅡ含量。结果:与模型组比较DD能显著提高心肌组织NO含量和结构型NOS(cNOS),Na+-K+-ATPase,Ca2+-ATPase活性;降低心肌组织诱生型NOS(iNOS)活性;抑制血浆及心肌组织AngⅡ和胶原的产生;减轻心脏质量参数(HW/BW,LVI)及MD。结论:DD对压力负荷性心肌肥厚大鼠心室重构具有保护作用;其机制与升高NO含量、抑制AngⅡ产生有关。
Objective: To investigate the protective effects of daidzein(DD) on ventricular remodeling in rats with myocardial hypertrophy induced by pressure overload and its mechanism.Method: Myocardial hypertrophy model of rats induced by pressure overload was prepared by constricting abdominal aorta.The operated rats were randomly divided into sham operated control group,aorta-constricted model group and three DD groups (30,60,120 mg·kg-1).Four weeks later,the heart-weight(HW),left ventricular weight(LVW),the ratio of HW/BW and LVW/BW(LVI) and the cardio-myocyte diameters(MD) after dyeing by HE colar were measured.The hydroxyroline,nitric oxide(NO) and the activity of nitric oxide synthetase(NOS) and Na+K+-ATPase,Ca2+-ATPase in left ventricle were quantified with spectrophotometry and the angiotensionⅡ(AngⅡ) in left ventricle and serum was messured with radioimmunoassay.Result: After treatment of the left ventricular with DD,vs aorta-contricted model group,NO content,cNOS and Na+-K+-ATPase,Ca2+-ATPase activity were significantly increased,the content of AngII in left ventricle and serum and iNOS activity and the ratio of HW/BW,LVI,MD were significantly reduced.Conclusion: DD has protective effects on ventricular remodeling in rats with myocardial hypertrophy induced by pressure overload and its mechanism may be related to raising NO content and reducing the level of AngⅡ.

关键词(KeyWords): 大豆苷元;心室重构;压力超负荷;一氧化氮;血管紧张素Ⅱ
daidzein(DD);myocardial hypertrophy;ventricular remodeling;pressure overload;nitric oxide(NO);angiotensionⅡ(AngⅡ)

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基金项目(Foundation): 江西省卫生厅中医药资助项目(2006A66)

作者(Author): 周俐;周青;熊小琴;刘建新;何蔚;
ZHOU Li,ZHOU Qing,XIONG Xiao-qing,LIU Jian-xin,HE Wei(Gannan Medical College,Ganzhou 341000,China)

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