中国中药杂志

2010, v.35(23) 3093-3096

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人工培植长春花中文多灵、长春质碱和长春碱的分布和积累动态
Distribution and accumulation of vindoline,catharanthine and vinblastine in Catharanthus roseus cultivated in China

于景华;原树生;庞海河;张学科;贾雪莹;唐中华;祖元刚;
YU Jinghua,YUAN Shusheng,PANG Haihe,ZHANG Xueke,JIA Xueying,TANG Zhonghua,ZU Yuangang(Key Laboratory of Forest Plant Ecology of Ministry of Education,Northeast Forestry University,Harbin 150040,China)

摘要(Abstract):

目的:研究田间栽培长春花不同发育阶段各器官文多灵、长春质碱和长春碱的含量动态以及不同器官的生物量动态,以确定人工种植长春花的最佳采收期。方法:以人工培植长春花为实验材料,采用高效液相色谱法对生长季内长春花根、茎、叶、花和果实中的文多灵、长春质碱和长春碱的含量进行动态检测。结果:这些生物碱含量受季节和组织部位的影响,叶片中文多灵和长春质碱含量最高,根中没有文多灵,花中长春碱含量最高;8-9月文多灵和长春质碱含量最高,9月以后长春碱含量最高;生物量9月初最高。结论:9月初为长春花最佳采收期。
Objective: The content of vindoline,catharanthine and vinblastine in the root,stem,leaf,flower and fruit of Catharanthus roseus at various developmental stages were determined,and the biomass allocation was also determined to find the best harvest time.Method: The content of vindoline,catharanthine and vinblastine in the root,stem,leaf,flower and fruit of C.roseus were determined by HPLC.Result: The content of these alkaloids were influenced by season and it varied in the different tissues of the plant.The content of vindoline and catharanthine in the leaves were the highest,and there was no vindoline detected in the root,but the content of vinblastine in the flower was the highest;the content of vindoline and catharanthine reached the maximum between the August and September,and the content of vinblastine reached the highest after the September.The biomass was the highest in the initial stage of September.Conclusion: The best harvest time was in the initial stage of September.

关键词(KeyWords): 长春花;发育阶段;文多灵;长春质碱;长春碱;采收期
Catharanthus roseus;developmental stages;vindoline;catharanthine;vinblastine;harvest time

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(30770338)

作者(Author): 于景华;原树生;庞海河;张学科;贾雪莹;唐中华;祖元刚;
YU Jinghua,YUAN Shusheng,PANG Haihe,ZHANG Xueke,JIA Xueying,TANG Zhonghua,ZU Yuangang(Key Laboratory of Forest Plant Ecology of Ministry of Education,Northeast Forestry University,Harbin 150040,China)

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