中国中药杂志

2016, v.41(11) 1978-1982

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芪蛭降糖胶囊对2型糖尿病大鼠肝组织中InsR,PI3K,GLUT2,p-JNK蛋白表达的影响
Effects of Qizhi Jiangtang capsule on protein expressions of InsR,PI3K,GLUT2 and p-JNK in hepatic tissues of rats with type 2 diabetes

刘博;于春江;孟祥宝;温富春;赵宏伟;孙桂波;丁涛;徐惠波;孙晓波;
LIU Bo;YU Chun-jiang;MENG Xiang-bao;WEN Fu-chun;ZHAO Hong-wei;SUN Gui-bo;DING Tao;XU Hui-bo;SUN Xiao-bo;Heilongjiang University of Chinese Medicine;Jilin Academy of Traditional Chinese Medicine;Jilin Yi Zheng Pharmaceutical Group Co.,Ltd.;The Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences;

摘要(Abstract):

观察芪蛭降糖胶囊对2型糖尿病大鼠降糖的效果,并探讨其降糖的初步作用机制。该实验采用高糖高脂配合小剂量链脲佐菌素(STZ)制备成2型糖尿病大鼠模型,连续给药6周后,检测各组动物血糖、糖化血清蛋白(GSP)水平;免疫组化法检测胰腺组织中胰岛β细胞的数量;蛋白印迹法检测肝组织中胰岛素受体(InsR)、磷脂酰肌醇-3激酶(PI3K)、葡萄糖转运体2(GLUT2)及磷酸化的c-Jun氨基末端激酶(p-JNK)蛋白的表达。结果显示,芪蛭降糖胶囊能够降低糖尿病动物的血糖和血清GSP水平,提升血清中胰岛素含量和胰岛β细胞的数量,提高肝组织中InsR,PI3K,GLUT2蛋白表达,降低p-JNK蛋白的表达。总之,芪蛭降糖胶囊降糖的作用较为稳定,其降糖机制可能与增加胰岛β细胞的数量而提升血清中胰岛素的含量,提高肝组织中InsR,PI3K,GLUT2蛋白表达并降低p-JNK蛋白表达从而减少肝组织的胰岛素抵抗有关。
To observe the hypoglycemic effect of Qizhi Jiangtang capsule in rats with type 2 diabetes,and investigate the preliminary mechanism of its hypoglycemic effect,type 2 diabetes rat models were established by high glucose and high fat combined with small dose of streptozotocin( STZ). After continuous administration for 6 weeks,blood glucose,and glycosylated serum protein( GSP) levels were detected in all of the animals; immunohistochemistry assay was used to detect the number of islet β cells; Western blot assay was used to detect the protein expression levels of insulin receptor( InsR),phosphoinositide-3 kinases( PI3K),glucose transporter-2( GLUT2) and phosphorylated Jun N-terminal kinases( p-JNK) in hepatic tissues. The results showed that Qizhi Jiangtang capsule could reduce the blood sugar and GSP levels in serum in animals with type 2 diabetes mellitus,increase the level of insulin in serum and number of islet β cells,increase the protein expression levels of InsR,PI3 K and GLUT2,and reduce the level of p-JNK protein expression. In conclusion,Qizhi Jiangtang capsule has relatively stable hypoglycemic effect,and the mechanism may be associated with increasing the number of islet β cells and level of insulin in serum,up-regulating the protein expression levels of InsR,PI3 K and GLUT2,down-regulating the level of p-JNK protein expression in hepatic tissues,and reducing the level of insulin in hepatic tissues.

关键词(KeyWords): 芪蛭降糖胶囊;2型糖尿病;胰岛素信号通路
Qizhi Jiangtang capsule;type 2 diabetes mellitus;insulin signaling pathway

Abstract:

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基金项目(Foundation): 国家“重大新药创制”科技重大专项(2011ZX09201-201-31,2012ZX09501001-004)

作者(Author): 刘博;于春江;孟祥宝;温富春;赵宏伟;孙桂波;丁涛;徐惠波;孙晓波;
LIU Bo;YU Chun-jiang;MENG Xiang-bao;WEN Fu-chun;ZHAO Hong-wei;SUN Gui-bo;DING Tao;XU Hui-bo;SUN Xiao-bo;Heilongjiang University of Chinese Medicine;Jilin Academy of Traditional Chinese Medicine;Jilin Yi Zheng Pharmaceutical Group Co.,Ltd.;The Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences;

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