中国中药杂志

2009, v.34(19) 2463-2465

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丹皮炭HPLC指纹图谱研究
HPLC fingerprints of Cortex Moutan Carbonisatum

李鹏;赵学龙;张丽;丁安伟;
LI Peng,ZHAO Xuelong,ZHANG Li,DING Anwei(Key Laboratory for Modern Research of Traditional Chinese Medical Formulae of Jiangsu,Nanjing University of Traditional Chinese Medicine,Nanjing 210046 China)

摘要(Abstract):

目的:建立丹皮炭的HPLC指纹图谱分析方法,为整体控制和评价丹皮炭的质量提供依据。方法:采用HederaODS-3柱(4.6 mm×150 mm,5μm),流动相甲醇-乙腈(1∶1)-0.5‰三氟乙酸水,梯度洗脱,检测波长258 nm。以丹皮酚为参照物,对10批丹皮炭进行指纹图谱的分析。结果:建立了丹皮炭饮片的HPLC指纹图谱共有模式,标定了11个共有峰,10批炭品的相似度在0.830~0.991。结论:该指纹图谱方法简便、准确、重复性好,可专属性地研究丹皮炭的指纹图谱,为有效地控制该饮片的内在质量提供了科学依据。
Objective: To establish the analytical method of the HPLC fingerprint of Cortex Moutan Carbonisatum and offer the evidence for quality control and quality evaluation of carbonized Cortex Moutan accordingly.Method: Ten batches of Cortex Moutan Carbonisatum were measured by HPLC with Paeonol as a reference substance and the HPLC analysis was performed on a Hedera ODS-3 Chromatographic Column(4.6 mm×150 mm,5 μm),with methanol to Acetonitrile(1∶1) and 0.5‰ trifluoroacetic acid solution as mobile phase in gradient mode and the detection wavelength was at 258 nm.Result: The common mode of the HPLC fingerprints were set up.There were 11 common peaks in the fingerprint of ten samples,and the similar degrees to the ten batches were between 0.830-0.991.Conclusion: The method is simple,accurate and have a good reaptability.And the quality of Cortex Moutan Carbonisatum can be controlled effectively by the HPLC fingerprint.

关键词(KeyWords): 丹皮炭;丹皮酚;指纹图谱;高效液相色谱法
Cortex Moutan Carbonisatum;paeonol;fingerprint;HPLC

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基金项目(Foundation): 国家“十一五”科技支撑计划项目(2006BAI09B006-02)

作者(Author): 李鹏;赵学龙;张丽;丁安伟;
LI Peng,ZHAO Xuelong,ZHANG Li,DING Anwei(Key Laboratory for Modern Research of Traditional Chinese Medical Formulae of Jiangsu,Nanjing University of Traditional Chinese Medicine,Nanjing 210046 China)

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