中国中药杂志

2008, v.33(24) 2946-2949

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三妙丸中牛膝对关节炎大鼠引药作用的机制研究
Study on drug-guide effect of Achyranthes bidentata in Sanmiao pill in arthritic rats

孙备;吕凌;陆忠祥;杨士友;
SUN Bei,LV Lin,LU Zhong-xiang,YANG Shi-you(Anhui Institute of Material Medica,Anhui Traditional Chinese Medicine Research and Development Key Laboratory, Hefei 230022,China)

摘要(Abstract):

目的:研究三妙丸中牛膝对关节炎大鼠引药作用的机制。方法:用弗氏完全佐剂和湿热处理法制备大鼠湿热痹症关节炎模型,造模成功的大鼠分为3组:模型组,含牛膝的三妙丸(18 g.kg-1)组,不含牛膝三妙丸(10 g.kg-1)组。HPLC法测定三妙丸中主要成分小檗碱在心脏、关节组织中的分布情况,体积法测定大鼠足关节肿胀度,同时观察组织病理变化;测定血浆血液流变学指标。结果:含牛膝三妙丸组在给药后2,4,6 h和多次给药14 d后关节浓度均高于不含牛膝组,关节/心脏小檗碱浓度比显著大于不含牛膝组。含牛膝的三妙丸明显改善关节炎模型大鼠的关节肿胀和组织病理改变。含牛膝三妙丸组明显改善关节炎大鼠血液流变学指标,与不含牛膝三妙丸组比P<0.01,与模型组相比也有显著性差异(P<0.01)。结论:牛膝能够引导三妙丸中主要有效成分小檗碱的靶组织分布,并与其药效作用相吻合。其促进小檗碱组织分布的作用和其活血作用可能有一定关系。
Objective:To study the drug-guiding mechanism of Achyranthes bidentata from Sanmiao pill in arthritic rats.Method: The rats were treated by Ferud's complet adjuvant and hot water-bath to establish rat adjuvant arthritis model,then the model rats were divided into three groups,model group,Sanmiao pill groups with A.bidentata.(18 g·kg-1)and without A.bidentata.(10 g·kg-1),respectively.The heart and foot joints were washed and homogenated to determine the berberine concentration by HPCL in different time after ig and the foot edema was tested by volume method.The pathological changes were observed and hemorheologic parameters were also tested.Result: The berberine concentration of foot joint was significantly higher in 2,4,6 hour and 14 day in the rats with A.bidentata.The berberine concentration ratio of foot joint and heart was significantly higher in rats with A.bidentata.pharmacodynamic researches showed that A.bidentata could enhance the edema inhibition effect of Sanmiao pill.Hemorheologic researches showed that A.bidentata.could sigmificantly improve the blood viscosity of model rats,the blood high shear viscosity,the blood low shear viscosity and the plasma viscosity were(6.47±0.57),(9.28±1.2),(1.94±0.19) mPa·s respectively.Conclusion: A.bidentata.could facilitate the targeted tissue distribution of berberine.The effect was correlative with its blood viscosity improvement.

关键词(KeyWords): 牛膝;三妙丸;引药;小檗碱;佐剂关节炎;组织分布
Achyranthes bidintata;Sanmiao pills;drug-guide;berberine;ferud's complet adjuvant arthritis;tissue distribution

Abstract:

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基金项目(Foundation): 安徽省自然科学基金项目(070413120)

作者(Author): 孙备;吕凌;陆忠祥;杨士友;
SUN Bei,LV Lin,LU Zhong-xiang,YANG Shi-you(Anhui Institute of Material Medica,Anhui Traditional Chinese Medicine Research and Development Key Laboratory, Hefei 230022,China)

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