袁明洋;黄必胜;余驰;刘义梅;陈科力;
YUAN Ming-yang;HUANG Bi-sheng;YU Chi;LIU Yi-mei;CHEN Ke-li;Key laboratory of Ministry of Education on Traditional Chinese Medicine Resource and Compound Prescription & Hubei University of Traditional Chinese Medicine;. The Xiantao Institute for Drug Control;

• 1:教育部中药资源和中药复方重点实验室&湖北中医药大学
• 2:仙桃市药品检验所

摘要(Abstract)：

该研究探索应用近红外光谱技术对8种含碳酸盐的矿物类中药进行快速鉴别。利用OPUS软件中聚类分析法建立的定性分析模型,可以准确鉴别8种含碳酸盐的矿物类中药。另外,使用偏最小二乘法对其中7种矿物类中药的主要成分碳酸钙含量建立近红外定量分析模型,以EDTA滴定法测定值为参照,当碳酸钙含量为47.61%⁹9.17%时,预测结果的平均相对偏差为0.24%,平均回收率为100.3%。结果表明,该近红外光谱分析模型可以快速准确鉴别8种含碳酸盐类矿物类中药,对其中7种矿物类中药中碳酸钙的含量也可以快速测定,结果准确、可靠。
The aim of this paper is to apply near infrared spectroscopy techniques to construct a rapid identification method for 8 kinds of mineral Chinese Medicines containing carbonates. The qualitative model using clustering analysis method in OPUS software can identify accurately 8 kinds of carbonate-containing mineral Chinese medicines. The near-infrared quantitative model was established by using partial least squares method( PLS) for 7 mineral Chinese Medicines in which main component is calcium carbonate. Compared with the results by EDTA titration,the established quantitative analysis model for calcium carbonate content showed a good prediction result that when the content is between 47. 61%-99. 17%,the average relative deviation of the prediction result is 0. 24% and the average recovery rate was 100. 3%. The results also showed that the model using near infrared spectroscopy can get not only a rapid identification of the 8 mineral Chinese medicines containing carbonates,but also an accurate and reliabe content determination of calcium carbonate for the 7 mineral Chinese medicines which contain the component.

关键词(KeyWords)： 近红外光谱法;碳酸盐;矿物类中药;聚类分析;碳酸钙;含量测定
near-infrared spectroscopy;carbonate;mineral Chinese medicines;cluster analysis;determination

Abstract:

Keywords:

基金项目(Foundation): 武汉市2012年高新技术产业发展行动计划生物技术与新医药专项(201260523193)

作者(Authors): 袁明洋;黄必胜;余驰;刘义梅;陈科力;
YUAN Ming-yang;HUANG Bi-sheng;YU Chi;LIU Yi-mei;CHEN Ke-li;Key laboratory of Ministry of Education on Traditional Chinese Medicine Resource and Compound Prescription & Hubei University of Traditional Chinese Medicine;. The Xiantao Institute for Drug Control;

参考文献(References)：

• [1]赵婷,高昂,巩江,等.鱼脑石药学研究概况[J].辽宁中医药大学学报,2011,13(9):82.
• [2]杨美华.石燕的矿物组分及微量元素的分析[J].江西中医学院学报,1997,9(1):33.
• [3]陈国佩,韦绍霞.钟乳石不同炮制品中碳酸钙含量测定[J].中国中医药科技,1996,3(6):25.
• [4]高娟,唐素芳,高立勤,等.浅谈近红外光谱技术在药物分析领域的应用[J].天津药学,2010,22(4):72.
• [5]许麦成.近红外漫反射光谱法快速测定地黄中梓醇含量[J].中药材,2011,34(7):1072.
• [6]高鸿彬,刘浩,相秉仁.半夏及其伪品天南星的近红外漫反射快速无损鉴别[J].光谱实验室,2012,29(2):899.
• [7]刘君动,叶正良,李德坤,等.近红外透射光谱法测定丹酚酸提取物中的丹酚酸B[J].光谱实验室,2012,29(2):1008.
• [8]杜敏,巩颖,乔延江,等.样品表面近红外光谱结合多类支持向量机快速鉴别枸杞子产地[J].光谱学与光谱分析,2013,33(5):1211.
• [9]蔡佳良,郭念欣,姬生国,等.运用近红外光谱法建立广藿香中百秋李醇的定量模型[J].中国中药杂志,2012,37(14):2113.
• [10]褚小立,袁洪福,陆婉珍.近红外分析中光谱预处理及波长选择方法进展与应用[J].化学进展,2004,16(4):528.
• [11]Cinier R,Guilment J.Quantitative analysis of resorcinol in aqueous solution by near-infrared spectroscopy[J].Bruker Report,1998,146:6.
• [12]Scafi S H F,Pasquini C.Identification of counterfeit drugs using near-infrared spectroscopy[J].Analyst,2001,126:2218.
• [13]徐广通,袁洪福,陆婉珍.现代近红外光谱技术及应用进展[J].光谱学与光谱分析,2000,20(2):134.
• [14]刘义梅,陈科力,袁明洋,等.近红外漫反射光谱法快速鉴别两组化石类中药材[J].世界科学技术——中医药现代化,2013,15(7):132.
• [15]白雁.现代近红外光谱分析技术在药品及食品品质评价系统中的应用[M].北京:高等教育出版社,2009:138.
• [16]严衍禄.近红外光谱分析基础与应用[M].北京:中国轻工业出社,2005:175.
• [17]胡玉清,赵中杰,江佩芬.中药石燕中碳酸钙和其他化学成分的测定[J].中国药学杂志,1989,24(7):421.
• [18]张晓敏.不同矿物药材重金属,有效成分,浸出物及有机质含量分析[D].西安:西北农林科技大学,2010.
• [19]夏振江,魏立新,杜玉枝,等.南寒水石质量标准研究[J].中南药学,2010,8(9):654.