中国中药杂志

1998, (09)

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楤木属植物总皂甙和齐墩果酸的含量测定
Determination of Oleanolic Acid and Total Saponins in Aralia L.

王玉萍,朱兆仪,王春兰,杨峻山
Wang Yuping,Zhu Zhaoyi,Wang Chunlan and Yang Junshan (Institute of Medicinal Plant,Chinese Academy of Medical Sciences ,Peking Union Medical College, Beijing 100094)

摘要(Abstract):

目的:从木属中寻找齐墩果酸和皂甙含量较高的资源植物,为用药提供科学依据。方法:木属植物根和茎中齐墩果酸和总皂甙的含量分别用高效液相色谱法和比色法测定。结果:被测的木属10种木本植物根和茎均含有齐墩果酸和皂甙,并以根或根皮中含量较高,4种草本植物含量极少或不含。结论:本属植物中黄毛木、辽东木、棘茎木、木、毛叶木的齐墩果酸含量较高,其中前两者的野生蕴藏量比较丰富,可以作为齐墩果酸的资源植物进行开发利用。
Objective:To search for higher contents of olenolic acid and saponins in Aralia for medicinal use.Method:A HPLC method was developed for the determination of olenolic acid in Aralia.An YWG C18 column was used and the mobile phase was methanolwater(90∶10).The detection wavelength was UV 210nm.The total saponins were determined by colorimetry.Result:Olenolic acid in root or stems of 13 species and total saponins in whole roots and root barks of 14 species of Aralia were determined.The contents of olenolic acid and total saponins in woody Aralia were higher than those in herbaceous.Conclusion:The contents of olenolic acid in A.decaisneana,A.elata,A.chinocaulis, A.chinensis and A.chinensis var.dasyphylloides are higher than those in other species.Furthermore,A.decaisneana and A.elata are rich in wild resources and thus of importance to the development and utilzation of wild plants containing olenolic acid. 

关键词(KeyWords): 楤木属,齐墩果酸,总皂甙,高效液相色谱法,比色法
Aralia;olenolic acid;total saponins;HPLC;colorimetry

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基金项目(Foundation): 自然科学基金

作者(Author): 王玉萍,朱兆仪,王春兰,杨峻山
Wang Yuping,Zhu Zhaoyi,Wang Chunlan and Yang Junshan (Institute of Medicinal Plant,Chinese Academy of Medical Sciences ,Peking Union Medical College, Beijing 100094)

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