中国中药杂志

2011, v.36(12) 1591-1595

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桃红四物汤抗氧化效应物质基础研究
Study on antioxidant effect and chemical constituents of Taohong Siwu decoction

刘立;段金廒;唐于平;马宏跃;宿树兰;李晓曦;
LIU Li,DUAN Jin′ao,TANG Yuping,MA Hongyue,SU Shulan,LI Xiaoxi(Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research,Nanjing University ofChinese Medicine,Nanjing 210046,China)

摘要(Abstract):

目的:评价桃红四物汤及各分离化学部位的抗氧化效应,对效应显著部位进行物质基础定性定量分析。方法:大孔吸附树脂分离制备各化学部位样品(TH-1~TH-15);采用清除DPPH和OH.的方法以及考察样品对过氧化氢(H2O2)损伤内皮细胞的保护作用,3种体外抗氧化实验评价桃红四物汤及各部位的抗氧化效应及特点。采用HPLC对效应显著部位的化学成分进行定性定量分析。结果:TH-2,TH-4,TH-7,TH-8,TH-9部位具有抗氧化效应,其中TH-8部位的抗氧化活性显著。分析结果表明TH-8部位中包含6种主要化学成分,分别为苦杏仁苷、芍药内酯苷、芍药苷、香豆酸、阿魏酸、苯甲酸,相对含量分别为75.70,31.26,60.79,1.196,1.108,4.861 mg·L-1。结论:一系列芳香酸及苷类成分是桃红四物汤抗氧化的主要效应物质基础。
Objective: To evaluate the antioxidant effects of Taohong Siwu decoction and to exploit the bioactive constituents.Method: The samples were prepared by macroporous adsorptive resins(TH-1-TH-15).Three antioxidant models were adopted to evaluate the antioxidant activities of Taohong Siwu decoction and its different separated fractions in vitro.It was found that fractions(TH-2,TH-4,TH-7,TH-8,TH-9),separated from Taohong Siwu decoction,mainly contributed to the antioxidant effects.The chemical constituents in the most active fraction TH-8 were identified and determined by HPLC.Result: TH-8 showed significant antioxidant activities in the antioxidant experiments.Six compounds in the fraction were determined which were amygdalin,albiflorin,paeoniflorin,benzoic acid,coumaric acid and ferulic acid.The contents were 75.70,31.26,60.79,1.196,1.108,4.861 mg L-1,respectively.Conclusion: Glycosides and aromatic acids may be the principle effective constituents in the active fraction.

关键词(KeyWords): 桃红四物汤;抗氧化;物质基础
Taohong Siwu decoction;antioxidant effects;bioactive constituents

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Keywords:

基金项目(Foundation): 国家科技支撑计划项目(2008BAI51B01);; 国家教育部“新世纪优秀人才支持计划”(NCET-09-0163);; 江苏省高校自然基金重大基础研究项目(06KJA36022,10KJA360039);; 江苏省方剂高技术研究重点实验室建设项目(BM2010576);; 江苏高校优势学科建设工程项目

作者(Author): 刘立;段金廒;唐于平;马宏跃;宿树兰;李晓曦;
LIU Li,DUAN Jin′ao,TANG Yuping,MA Hongyue,SU Shulan,LI Xiaoxi(Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research,Nanjing University ofChinese Medicine,Nanjing 210046,China)

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