中国中药杂志

2022, v.47(18) 4895-4907

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苍术根茎转录组比较分析及倍半萜生物合成基因发掘
Comparison of transcriptome of Atractylodes lancea rhizome and exploration of genes for sesquiterpenoid biosynthesis

曹也;张文晋;常丽坤;康传志;王月枫;谢冬梅;王升;郭兰萍;
CAO Ye;ZHANG Wen-jin;CHANG Li-kun;KANG Chuan-zhi;WANG Yue-feng;XIE Dong-mei;WANG Sheng;GUO Lan-ping;School of Pharmacy, Anhui University of Chinese Medicine;State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences;School of Traditional Chinese Medicine, Guangdong Pharmaceutical University;

摘要(Abstract):

为了研究苍术根茎不同生长发育时期转录水平差异,发掘其中倍半萜类化合物生物合成途径相关酶编码基因,对3个不同生长发育时期苍术根茎进行了转录组测序及比较分析。利用Illumina NovaSeq 6000技术对生长期(SZ)、开花期(KH)和采收期(CS)的苍术根茎进行了cDNA文库测序。共得到388 201 748条clean reads,比较SZ vs KH、KH vs CS、SZ vs CS,分别鉴定到16 925、8 616、13 702条差异表达基因(DEGs),注释到53条可能参与到倍半萜生物合成途径中的基因,其中甲羟戊酸(MVA)途径上注释到可能编码6种酶的9条基因,2-C-甲基-D-赤藓糖醇-4-磷酸(MEP)途径中注释到可能编码7种酶的15条基因,倍半萜和三萜生物合成代谢通路中注释到29条基因。通过基因共表达网络分析(WGCNA)得到与生长期、开花期和采收期相关的倍半萜类化合物生物合成关键基因分别有12、1、1条,再联合共表达网络图挖掘到多个倍半萜类化合物合成候选基因。该研究为探索研究苍术根茎中倍半萜类化合物的生物合成途径并解析其表达调控与倍半萜类物质累积的分子机制奠定了基础。
This study compared the transcriptome of Atractylodes lancea rhizome at different development stages and explored genes encoding the key enzymes of the sesquiterpenoid biosynthesis pathway. Specifically, Illumina NovaSeq 6000 was employed for sequencing the cDNA libraries of A. lancea rhizome samples at the growth stage(SZ), flowering stage(KH), and harvesting stage(CS), respectively. Finally, a total of 388 201 748 clean reads were obtained, and 16 925, 8 616, and 13 702 differentially expressed genes(DEGs) were identified between SZ and KH, KH and CS, and SZ and CS, separately. Among them, 53 genes were involved in the sesquiterpenoid biosynthesis pathways: 9 encoding 6 enzymes of the mevalonic acid(MVA) pathway, 15 encoding 7 enzymes of the 2-C-methyl-D-erythritol-4-phosphate(MEP) pathway, and 29 of sesquiterpenoid and triterpenoid biosynthesis pathway. Weighted gene co-expression network analysis(WGCNA) yielded 12 genes related to sesquiterpenoid biosynthesis for the SZ, 1 gene for the KH, and 1 gene for CS, and several candidate genes for sesquiterpenoid biosynthesis were discovered based on the co-expression network. This study laid a solid foundation for further research on the sesquiterpenoid biosynthesis pathway, analysis of the regulation mechanism, and mechanism for the accumulation of sesquiterpenoids in A. lancea.

关键词(KeyWords): 苍术根茎;倍半萜生物合成;转录组分析
Atractylodes lancea rhizome;sesquiterpenoid biosynthesis;transcriptome analysis

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金重大项目(81891014);; 中国中医科学院科技创新工程项目(CI2021A03903)

作者(Authors): 曹也;张文晋;常丽坤;康传志;王月枫;谢冬梅;王升;郭兰萍;
CAO Ye;ZHANG Wen-jin;CHANG Li-kun;KANG Chuan-zhi;WANG Yue-feng;XIE Dong-mei;WANG Sheng;GUO Lan-ping;School of Pharmacy, Anhui University of Chinese Medicine;State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences;School of Traditional Chinese Medicine, Guangdong Pharmaceutical University;

DOI: 10.19540/j.cnki.cjcmm.20220615.101

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