淡墨;高先富;谢国祥;刘忠;赵爱华;贾伟;
DAN Mo,GAO Xian-fu,XIE Guo-xiang,LIU Zhong,ZHAO Ai-hua,JIA Wei(School of Pharmacy,Shanghai Jiao Tong University,Shanghai 200240,China)

• 1:上海交通大学药学院
• 2:上海交通大学药学院
• 3:上海交通大学药学院
• 4:上海交通大学药学院

摘要(Abstract)：

代谢组学作为系统生物学的重要分支,近年来在植物研究领域受到广泛关注,并取得了重要进展。植物代谢组学目前虽然是一个并不成熟的领域,但是在研究模式植物的生理生化过程和基因改造等方面已成为最为有效的手段之一,近年在研究非模式植物方面特别是药用植物方面也取得了突破性进展。作者在总结植物代谢组学的定义和发展的基础上重点阐述了其最新应用,并提出了植物代谢组学可能的发展方向。植物代谢组学为植物研究提供了一个整体全面的分析平台,从宏观角度更加科学地理解植物生理生化过程,进而为人类所用提供了可能。许多植物代谢物是中药的活性成分,其种类和含量随品种、生长环境、采集季节以及炮制方法等因素而变化。因此,中药的质量问题主要就是植物代谢组的问题。由于环境因素、植物有效部位对中药药效(或价值)有一定影响,而代谢组学法可以有效检测这些因素,植物代谢组学为深入研究开发中药资源创造了新的研究平台。
Metabolomics,a branch of systems biology, has gained extensive attention and profound achievements in the plant.Although plant metabolomics is to be explored,it has been one of the most effective methods to study the physiological and biochemical process and gene modification in pattern plants.We herein summarized the concept,development,and application of metabolomics and prospected the potentials in the metabolite profiling for plant.Metabolomics provides an omics' methodology to elucidate the whole biological process,identify and quantify the complex components in the plant.A number of metabolites present in the plant are active components of traditional Chinese medicine,and these bioactive components are influenced by the multi-factors such as environment,species,and processing methods etc.Therefore,it is of great importance to analyze a wide spectrum of compositions with diverse chemical characteristics and varied concentration,which is the foundation to quality control,allowing the elucidation of the pharmacological effectiveness,and further exploiting of traditional Chinese medicine.

关键词(KeyWords)： 代谢组学;代谢途径;系统生物学;中药;功能基因组学;蛋白质组学;转录组学
metabolomics;metabolic pathway;traditional Chinese medicine;system biology;functional genomics;proteomics;transcriptomics;medicinal plants

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 淡墨;高先富;谢国祥;刘忠;赵爱华;贾伟;
DAN Mo,GAO Xian-fu,XIE Guo-xiang,LIU Zhong,ZHAO Ai-hua,JIA Wei(School of Pharmacy,Shanghai Jiao Tong University,Shanghai 200240,China)

参考文献(References)：

• [1]Tweeddale H,Notley-McRobb L,Ferenci T.Effect of slowgrowth on metabolism ofEscherichia coli,as revealed by globalmetabolite pool(‘Metabolome’)analysis[J].J Bacteriol,1998,180:5109.
• [2]Trethewey R N,Krotzky A J,Willmitzer L.Metabolic profiling:a Rosetta stone for genomics[J].Curr Opin Plant Biol,1999,2:83.
• [3]Fiehn O,Kopka J,Dormann P,et al.Metabolite profiling forplant functional genomics[J].Nat Biotechnol,2000,18:1157.
• [4]Dixon R A,Strack D.Phytochemistry meets genome analysis,and beyond[J].Phytochemistry,2003,62:815.
• [5]Bino R J,Hall R D,Fiehn O,et al.Potential of metabolomicsas a functional genomics tool[J].Trends Plant Sci,2004,9:418.
• [6]Von Roepenack-Lahaye E,Degenkolb T,Zerjeski M,et al.Pro-filing of Arabidopsis secondary metabolites by capillary liquidchromatography coupled to electrospray ionization quadrupoletime-of-flight mass spectrometry[J].Plant Physiol,2004,134:548.
• [7]Facchini P J,Bird D A,St-Pierre B.CanArabidopsismake com-plex alkaloids[J].Trends Plant Sci,2004,9:116.
• [8]Aharoni A,Ric de Vos CH,Verhoeven HA,et al.Nontargetedmetabolome analysis by use of Fourier transform ion cyclotronmass spectrometry[J].Omics,2002,6:217.
• [9]Sato S,Soga T,Nishioka T,Tomita M.Simultaneous determina-tion of main metabolites in rice leaves using capillary electropho-resis mass spectrometry and capillary electrophoresis diode arraydetection[J].Plant J,2004,40:151.
• [10]Goodacre R,Vaidyanathan S,Dunn W B,et al.Metabolomicsby numbers:acquiring and understanding global metabolomicsdata[J].Trends Biotechnol,2004,2:245.
• [11]魏陆海,孙维洋.代谢组学与中药现代研究[J].山东中医药大学学报,2006,30(4):336.
• [12]宋晓亭,胡惠平,安红梅.数字化色谱指纹谱技术描述复方专利技术特征中的若干问题的探讨[J].世界科学技术———中医药现代化,2005,7(3):824.
• [13]Fiehn O.Metabolic networks of Cucurbita maxima phloem[J].Phytochemistry,2003,62(6):875.
• [14]Kin HK,Choi Y H,Erkelens C,et al.Metabolic fingerprintingofEphedraspecies using1H-NMR spectroscopy and principalcomponent analysis[J].Chem Pharm Bull,2005,53(1):105.
• [15]齐炼文,李萍,赵静.代谢组学与中医药现代研究[J].世界科学技术———中医药现代化,2006,8(6):79.
• [16]Murch S J,Rupasinghe HP,Goodenowe D,et al.A metabolo-mic analysis of medicinal diversity in Huang-qin(Scutellaria ba-icalensisGeorgi)genotypes:discovery of novel compounds[J].Plant Cell Rep,2004,23(6):419.
• [17]Davuluri G R,van Tuinen A,Fraser P D,et al.Fruit-specificRNAi-mediated suppression of DET1 enhances carotenoid andflavonoid content in tomatoes[J].Nat Biotechnol,2005,23:890.
• [18]Fukusaki E,Jumtee K,Bamba T,et al.Fingerprinting and pro-filing of Arabidopsis thaliana leaf and its cultured cells T87 byGC/MS[J].Z Naturforsch,2006,61(3-4):267.
• [19]Hirai MY,Yano M,Goodenowe D B,et al.Integration of tran-scriptomics and metabolomics for understanding of global respon-ses to nutritional stresses inArabidopsis thaliana[J].Proc NatlAcad Sci,2004,101,10205.
• [20]Hirai MY,Saito K.Post-genomics approaches for the elucidationof plant adaptive mechanisms to sulphur deficiency[J].J ExpBot,2004,55(404):1871.
• [21]Corey D,Broeckling D V,Huhman MA,et al.Metabolic profi-ling ofMedicago truncatulacell cultures reveals the effects of bi-otic and abiotic elicitors on metabolism[J].J Exp Bot,2005,56(410):323.
• [22]Maier W,Schmidt J,Wray V,et al.The arbuscular mycorrhizalfungus,Glomus intraradices,induces the accumulation of cyclo-hexenone derivatives in tobacco roots[J].Planta,1999,207,620.
• [23]Aharoni A,de Vos chr,Verhoeven H A,et al.Nontargetedmetabolome analysis by use of Fourier Transform Ion CyclotronMass Spectrometry[J].OMICS,2002,6:217.
• [24]Rolleston F S.A theoretical background to the use of measuredintermediates in the study of the control of intermediary metabo-lism[J].Curr Top Cell Regul,1972,5:47.
• [25]Tiessen A,Hendriks J H M,Stitt M,et al.Starch synthesis inpotato tubers is regulated by post-translational redox modificationof ADP-glucose pyrophosphorylase:a novel regulatory mecha-nism linking starch synthesis to the sucrose supply[J].PlantCell,2002,14:2191.
• [26]Stitt M,Sonnewald U.Regulation of metabolism in transgenicplants[J].Ann Rev Plant Physio Plant Molec Biol,1995,46:341.
• [27]Stitt M.Nitrate regulation of metabolism and growth[J].CurrOpin Plant Biol,1999,2:178.
• [28]Urbanczyk-Wochniak E.Profiling of diurnal patterns of metabo-lite and transcript abundance in potato(Solanum tuberosum)leaves[J].Planta,2005,221(6):8913.
• [29]Morreel K,Goeminne G,Storme V,et al.Genetical metabolo-mics of flavonoid biosynthesis in Populus:a case study[J].Plant J,2006,47(2),224.
• [30]Keurentjes J J,Fu J,de Vos C H,et al.The genetics of plantmetabolism[J].Nat Genet,2006,38(7):842.
• [31]Schauer N,Semel Y,Roessner U,et al.Comprehensive meta-bolic profiling and phenotyping of interspecific introgression linesfor tomato improvement[J].Nat Genet,2006,24(4):447.
• [32]Sarry J E,Kuhn L,Ducruix C,et al.The early responses ofAr-abidopsis thalianacells to cadmium exposure explored by proteinand metabolite profiling analyses[J].Proteomics,2006,6:2180.
• [33]Hirai MY,Klein M,Fujikawa Y,et al.Elucidation of gene-to-gene and metabolite-to-gene networks inarabidopsisby integra-tion of metabolomics and transcriptomics[J].J Biol Chem,2005,280(27):25590.
• [34]Ferrario-Mery S,Hodges M,Hirel B,et al.Photorespiration de-pendent increases in phosphoenolpyruvate carboxylase,isocitratedehydrogenase and glutamate dehydrogenase in tobacco plantsdeficient inferredoxin-dependent glutamine-aketoglutarate aminotransferase[J].Planta,2002,214:877.
• [35]Rischer H,Oresic M,Seppanen-Laakso T,et al.Gene-to-me-tabolite networks for terpenoid indole alkaloid biosynthesis inCatharanthus roseuscells[J].Proc Natl Acad Sci,2006,103(14):5614.