中国中药杂志

2015, v.40(09) 1738-1743

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含量测定和指纹图谱结合LC-MS技术整体评价银杏叶片的质量
Quantitative and qualitative evaluation on tablets of Ginkgo biloba leaves using fingerprint and LC-MS analysis

梁文琳;谢达温;丁岗;徐殿红;孙永成;廉源沛;李艳静;萧伟;
LIANG Wen-lin;XIE Da-wen;DING Gang;XU Dian-hong;SUN Yong-cheng;LIAN Yuan-pei;LI Yan-jing;XIAO Wei;Jiangsu Kanion Pharmaceutical Co. ,Ltd.;China Pharmaceutical University;

摘要(Abstract):

建立银杏叶片合理质量控制的方法,评价市售不同厂家银杏叶片的质量。收集市售银杏叶片,采用HPLC对其进行总黄酮醇苷和萜类内酯含量测定,根据2010年版《中国药典》一部方法进行测定,2批银杏叶片总黄酮醇苷含量不合格,3批银杏叶片萜类内酯含量不合格;建立HPLC指纹图谱检测方法对其进行检测,不同厂家生产的16批银杏叶片HPLC指纹图谱标记出31个共有峰,与生成的对照指纹图谱相似度为0.763~0.989;采用LC-MS对指纹图谱色谱峰进行指认,鉴定出31个黄酮类成分。以总黄酮醇苷含量、萜类内酯含量和指纹图谱结合LC-MS技术整体评价银杏叶片的质量,为银杏叶片质量研究提供一些研究思路。
A reasonable method for the quality control of tablets of Ginkgo biloba leaves was established in this paper. The total flavonol glycosides and terpene lactones of G. biloba tablets were quantified by HPLC. Totally,16 batches of the commercially available tablets of G. biloba leaves were determined. Among of them,2 batches were unqualified in the content of total flavonol glycosides,and3 batches were unqualified in the content of terpene lactones. A validated HPLC fingerprint method was established to evaluate the commercially available tablets of G. biloba leaves with the assistance of LC-MS. Sixteen batches showed the similarity of 0. 763-0. 989.There were 31 fingerprint chromatogram peaks were identified as flavonoids compositions by LC-MS. This provides a research idea for the quality control of tablets of G. biloba leaves.

关键词(KeyWords): 银杏叶片;银杏叶标准提取物;总黄酮醇苷;指纹图谱;LC-MS;质量评价
Ginkgo biloba leaves tablets;ginkgo leaves extracts;total flavonol glycosides;fingerprint;LC-MS;quality evaluation

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基金项目(Foundation): 国家科技支撑计划项目(2011BA04B03)

作者(Author): 梁文琳;谢达温;丁岗;徐殿红;孙永成;廉源沛;李艳静;萧伟;
LIANG Wen-lin;XIE Da-wen;DING Gang;XU Dian-hong;SUN Yong-cheng;LIAN Yuan-pei;LI Yan-jing;XIAO Wei;Jiangsu Kanion Pharmaceutical Co. ,Ltd.;China Pharmaceutical University;

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