中国中药杂志

2010, v.35(15) 1947-1952

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基于HPLC-DAD-ESI-MS技术的芍药甘草汤体内外物质基础变化研究
Studies on changes of in vitro and in vivo material base of Shaoyao Gancao decoction based on HPLC-DAD-ESI-MS

沈岚;冯怡;徐德生;林晓;洪燕龙;王优杰;张梁;
SHEN Lan1,2,FENG Yi1,2,XU Desheng3,LIN Xiao1,2,HONG Yanlong1,WANG Youjie1,ZHANG Liang1(1.Engineering Research Center of Modern Preparation Technology of Traditional Chinese Medicine,Ministry of Education,Shanghai 201203,China;2.School of Pharmacy,Shanghai university of Traditional Chinese Medicine,Shanghai 201203,China;3.Shuguang Hospital,Shanghai university of Traditional Chinese Medicine,Shanghai 200021,China)

摘要(Abstract):

目的:采用HPLC-DAD-ESI-MS分析芍药甘草配伍体内外物质基础变化,确认大鼠血中移行组分。方法:以Kor-masilC18色谱柱(4.6mm×250mm,5μm带保护柱);以乙腈-0.5%乙酸水为流动相进行梯度洗脱,流速0.8mL·min-1,柱温25℃,以甲醇为去蛋白溶剂,样品经电喷雾离子源正离子化后,通过LCQdeca型质谱仪,在m/z100~1000进行扫描,并对特征离子进行2次裂解,获得二级质谱数据。结果:以芍药甘草复方组方前后的体外成分研究为基础,确认了芍药甘草汤体外的主要成分为芍药苷、儿茶素-5-O-葡萄糖苷、白芍苷、甘草苷、甘草酸等,入血移行成分中也发现此类糖苷类物质,另又发现了来源于甘草成分的葡萄糖醛酸结合物——甘草素葡萄糖醛酸和异甘草酸葡萄糖醛酸。结论:采用液相色谱-质谱联用技术分析芍药甘草汤大鼠血中移行组分,并与芍药甘草复方组方前后的成分进行对比,可对复方入血的成分进行推测和确认,具有简便、快速的优点,且对于中药复方物质基础研究具有较好的应用价值。
Objective:To study the changes of the in vitro and in vivo material base of Shaoyao Gancao decoction(SGD)based on HPLC-DAD-ESI-MS,and confirm the migrational constituents in plasma.Method:Kormasil C18(4.6 mm×250 mm,5 μm with protection column)was employed;acetonitrile and 0.5% acetic acid/water was used as mobile phase for gradient elution,flow rate was set at 0.8 mL·min-1,column temperature was 25 ℃,and methanol was used to remove the protein.A LCQdeca mass spectrometer system equipped with an electrospray ionization iontrap source was used as the detector and operated in the positive ion mode.Ions was scanned from the m/z 100 to m/z 1 000,and characteristic ion were secondary schizolysised to obtain data of second order MS.Result:The main constitutes of SGD were peoniflorin,Catechin-5-O-glucoside,albiflorin,liquiritin and glycyrrhizic acid etc,and glucosides were found in the plasma samples of rats administered with SGD,which were glucuronide conjunctions of liquiritigenin and isoliquiritigenin.Conclusion:Liquid chromatography-mass spectrometry technology provides a convenient and high speed method to analyze the migrational constituents of plasma of rats administered with SGD,therefore,it has a good value in studies on the changes in the material base of traditional Chinese medicine(TCM).

关键词(KeyWords): 高效液相色谱-质谱联用;芍药甘草汤;物质基础;葡萄糖醛酸结合物
HPLC-DAD-ESI-MS;Shaoyao Gancao decoction;material base;glucuronide conjunction

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金青年科学基金项目(30801548);; 上海市青年科技启明星计划(08QA14060);; 国家教育部博士点专项基金(20060268009);; 上海市教委重点学科项目(J50302);; 上海市自然科学基金(09ZR1429600);; 上海市教委科研创新项目(10YZ57)

作者(Author): 沈岚;冯怡;徐德生;林晓;洪燕龙;王优杰;张梁;
SHEN Lan1,2,FENG Yi1,2,XU Desheng3,LIN Xiao1,2,HONG Yanlong1,WANG Youjie1,ZHANG Liang1(1.Engineering Research Center of Modern Preparation Technology of Traditional Chinese Medicine,Ministry of Education,Shanghai 201203,China;2.School of Pharmacy,Shanghai university of Traditional Chinese Medicine,Shanghai 201203,China;3.Shuguang Hospital,Shanghai university of Traditional Chinese Medicine,Shanghai 200021,China)

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