中国中药杂志

2015, v.40(07) 1370-1375

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何首乌水提物对大鼠肝脏CYP1A2,CYP2E1酶活性及mRNA表达抑制作用研究
Study on inhibitory effect of water extract of Polygonum multiflorum on CYP1A2 and CYP2E1 enzymatic activities and mRNA expressions in rat liver

李浩;杨红莉;李登科;冯光远;魏宝红;张园园;张玉杰;孙震晓;
LI Hao;YANG Hong-li;LI Deng-ke;FENG Guang-yuan;WEI Bao-hong;ZHANG Yuan-yuan;ZHANG Yu-jie;SUN Zhen-xiao;College of Traditional Chinese Medicine,Beijing University of Chinese Medicine;

摘要(Abstract):

大鼠连续灌胃不同剂量的何首乌水提物(1,10 g·kg~(-1))7 d,取肝脏制备肝微粒体,分别用Cocktail体外孵育法和实时荧光定量PCR技术观察何首乌水提物对大鼠肝脏主要CYP450酶活性及mRNA表达的影响。与空白对照组相比,1,10 g·kg~(-1)何首乌水提物组大鼠肝脏CYP2E1酶活性和mRNA的表达均受到明显抑制(CYP2E1酶活性,P<0.01;CYP2E1的mRNA表达1 g·kg~(-1)组P<0.05,10 g·kg~(-1)组P<0.01),CYP3A1酶活性虽显示明显升高(P<0.01),但mRNA的表达没有显著变化;10 g·kg~(-1)何首乌水提物组大鼠肝脏CYP1A2酶活性和mRNA的表达受到明显抑制(P<0.01)。
Rats were continuously given different doses of water extract of Polygonum multiflorum(1,10 g·kg~(-1)) for 7 days to prepare liver microsomes.Cocktail in vitro incubation approach and Real-time quantitative PCR technology were used to observe the effect of water extract of P.multiflorum on CYP450 enzymatic activities and mRNA expressions in rat liver.Compared with the blank control group,both 1,10 g·kg~(-1) water extract of P.multiflorum treated groups showed significant inhibitions in CYP2E1 enzymatic activities and mRNA expressions(enzymatic activities of CYP2E1,P<0.01;mRNA expression of CYP2E1,P<0.05 in 1 g·k~(-1)group,P < 0.01 in 10 g·kg~(-1)group).They revealed a significant increase in the enzymatic activity of CYP3A1(P < 0.01),but without significant change in mRNA expressions.The 10 g·kg~(-1) group showed a significant inhibition in CYP1A2 enzymatic activities and mRNA expressions in rat livers(P < 0.01).

关键词(KeyWords): 何首乌水提物;CYP1A2;CYP2E1;CYP3A1;大鼠肝微粒体;Cocktail探针法;mRNA表达
water extract of Polygonum multiflorum;CYP1A2;CYP2E1;CYP3A1;rat liver microsome;Cocktail probe approach;mRNA expression

Abstract:

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基金项目(Foundation): 国家自然科学基金项目(81473418);; 国家林业局野生动植物保护项目(2012-2014);; 北京中医药大学自主选题项目(2013JYBZZXS-088,2014JYBZZ-XS-097)

作者(Author): 李浩;杨红莉;李登科;冯光远;魏宝红;张园园;张玉杰;孙震晓;
LI Hao;YANG Hong-li;LI Deng-ke;FENG Guang-yuan;WEI Bao-hong;ZHANG Yuan-yuan;ZHANG Yu-jie;SUN Zhen-xiao;College of Traditional Chinese Medicine,Beijing University of Chinese Medicine;

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