张彦华;唐于平;郭建明;丁安伟;段金廒;
ZHANG Yanhua,TANG Yuping*,GUO Jianming,DING Anwei*,DUAN Jinao (Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research,Nanjing University of Chinese Medicine,Nanjing 210046,China)

• 1:南京中医药大学江苏省方剂研究重点实验室

摘要(Abstract)：

目的:探讨5个活血化瘀方(血府逐瘀汤、少腹逐瘀汤、膈下逐瘀汤、身痛逐瘀汤、通窍活血汤)对家兔血小板聚集及凝血酶时间的作用及其量效关系。方法:采用比浊法测定ADP诱导家兔血小板聚集率和凝血酶时间(TT)法测定家兔血浆凝血时间。结果:活血化瘀方能显著抑制ADP诱导的家兔血小板聚集、延长凝血时间,5个方剂醇沉上清液部位抗血小板聚集效果比其全方和醇沉沉淀部位效果好(P<0.01),其中少腹逐瘀汤和膈下逐瘀汤醇沉上清液部位抗血小板聚集效果比其他方剂好,而在延长家兔血浆凝血时间方面全方效果最好(P<0.01),醇沉上清液部位效果最差。结论:5个活血化瘀方对ADP诱导血小板聚集和凝血酶时间均有显著影响,且不同部位样品侧重的药效强弱不同。通过本实验研究为进一步的方剂活血化瘀整体评价奠定了基础,为中医临床治疗心脑血管疾病提供了科学依据。
Objective: To investigate the effect of five traditional Chinese medicine formula for promoting blood circulation to remove stasis(Xuefuzhuyu Tang,Shaofuzhuyu Tang,Gexiazhuyu Tang,ShentongZhuyu Tang,Tongqiaohuoxue Tang) on platelet aggregation and clotting time in a dose-response relationship.Method: The platelet aggregation was tested with Born's method and thrombin time(TT) method was established to determine the clotting time.Result: The formula for promoting blood circulation to remove stasis showed significant inhibitory effects on the platelet aggregation and prolonged clotting time,the supernate of 80% alcohol solution showed the most potent inhibitory activity among the three kinds of samples from one formula(P<0.01),especially the supernate of Shaofuzhuyu Tang and Gexiazhuyu Tang were better than other formula on platelet aggregation.About the anticoagulant,the whole formula showed the evidently prolonging the clotting time than other two fractions(P<0.01) and the precipitation were the worst.Conclusion: The formula for promoting blood circulation to remove stasis showed significant effect on platelet aggregation and clotting time,and different samples of the formula had different efficacy.The study established a foundation for further bio-activity evaluation of these formulae and provided evidence for the treatment of cardiovascular and cerebrovascular diseases.

关键词(KeyWords)： 活血化瘀方;血小板聚集;ADP;凝血酶时间
Formulae for promoting blood circulation to remove stasis;platelet aggregation;ADP;TT

Abstract:

Keywords:

基金项目(Foundation): 江苏高校自然科学重大基础研究项目(06KJA36022,07KJA36024)

作者(Authors): 张彦华;唐于平;郭建明;丁安伟;段金廒;
ZHANG Yanhua,TANG Yuping*,GUO Jianming,DING Anwei*,DUAN Jinao (Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research,Nanjing University of Chinese Medicine,Nanjing 210046,China)

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