中国中药杂志

2015, v.40(20) 4026-4030

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反相离子对色谱法测定三七中三七素含量
Content determination of dencichine in Panax Notoginseng by a reversed phase ion-pair chromatography

李琳;王承潇;曲媛;崔秀明;
LI Lin;WANG Cheng-xiao;QU Yuan;CUI Xiu-ming;Faculty of Life Science and Technology,Kunming University of Science and Technology;

摘要(Abstract):

建立反相离子对色谱法测定三七中三七素含量。通过超声提取样品中的三七素,并采用四丁基氢氧化铵离子对试剂与反相高效液相色谱法结合直接测定三七素含量。确定超声提取优化条件为物液比为1∶20,连续超声提取2次,每次15min,3 500 r·min-1,离心15 min。三七不同生长年限、产地、部位、以及同属植株中三七素含量均有差异:一年生三七茎叶三七素含量最高;红河州样品三七素含量高于文山州;真空冷冻干燥方式优于其他加工方式;三七高于其他同属植株。该检测方法操作简便,分离度好,稳定性好,适用于三七素含量直接测定,为三七中三七素的质量标准控制提供参考。
To build a reversed phase ion-pair chromatography to determination content of Dencichine from Panax notoginseng.Using Tetrabutyl ammonium hydroxide ions by the combination of reagent and HPLC method without derivatization to test the content of dencichine directly. The optimum conditions of supersonic extraction were solid-to-liquid ratio 1∶ 20,Continuous ultrasonic extraction:twice,each time 15 minutes,3 500 r·min-1,then centrifuging 15 minutes. Dencichine in different age,place,part and the different Processing mode were examined. The method is simple with sound separation degree and stability,which can facilitate the determination of dencichine content directly and provide the basis in quality standard of raw material.

关键词(KeyWords): 三七;三七素;高效液相色谱法;离子对试剂;含量测定
Panax notoginseng;dencichine;HPLC;ion-pair reagent;determination content

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作者(Author): 李琳;王承潇;曲媛;崔秀明;
LI Lin;WANG Cheng-xiao;QU Yuan;CUI Xiu-ming;Faculty of Life Science and Technology,Kunming University of Science and Technology;

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