中国中药杂志

2016, v.41(24) 4621-4627

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

山西产苦参花中生物碱类和黄酮类成分的分析与评价
Analysis and evaluation of alkaloids and flavonoids in flower of Sophora flavescens from Shanxi province

张黄琴;朱振华;钱大玮;翁泽斌;郭盛;段金廒;雷振宏;李安平;
ZHANG Huang-qin;ZHU Zhen-hua;QIAN Da-wei;WENG Ze-bin;GUO Sheng;DUAN Jin-ao;LEI Zhen-hong;LI An-ping;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,State Administration of Traditional Chinese Medicine Key Laboratory of Chinese Medicinal Resources Recycling Utilization,National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Nanjing University of Chinese Medicine;Shanxi Zhen Dong

摘要(Abstract):

该研究以苦参花资源化利用为目的,分析苦参花不同物候期中生物碱类、黄酮类资源性化学成分的含量,以期发现苦参花资源的潜在利用价值。采用UPLC-TQ-MS联用技术,对山西长治产苦参花中7个生物碱类及7个黄酮类成分进行分析测定。结果显示不同花期中都含有金雀花碱、氧化苦参碱、氧化槐果碱、槐定碱、N-甲基金雀花碱、苦参碱、槐果碱7种生物碱,各碱总和为花蕾1.47%、半开花1.34%、盛开花1.17%、败花1.01%,其中主要生物碱为N-甲基金雀花碱、氧化槐果碱、氧化苦参碱,约占总生物碱量的83%;所测定的芦丁、木犀草素、槲皮素、异槲皮苷、三叶豆紫檀苷、苦参酮、苦参醇7种黄酮类成分,各黄酮总和花蕾495.2μg·g-1、半开花313.7μg·g-1、败花224.2μg·g-1、盛开花193.0μg·g-1,其中木犀草素的含量相对较高,约占所测总黄酮量的89%~94%。结果提示苦参花所含的喹诺里西啶类生物碱是其主要的资源性化学成分,可加以开发利用。
This study intends to explore the potential resource-orientedutilization value of the flower of Sophora flavescents by analyzing alkaloids and flavonoids in the flower of S. flavescens from Shanxi province. This study established a rapid UPLC-TQ-MS/MS method that is used for determination of seven alkaloids and seven flavonoids in the flower of S. flavescens. The different florescences all have the seven detected alkaloids such as cytisine,oxy-matrine,oxy-sophocarpine,sophoridine,N-methylcytisine,matrine,sophocarpine. The total contents of detected alkaloids are as follows: flower buds 1. 47%,primal flowers 1. 34%,full bloomed flowers1. 17%,faded flowers 1. 01%. The top three contents of alkaloids are N-methylcytisine,oxy-sophocarpine and oxymatrine,accounting for about 83% of the total amount of detected alkaloids. All the samples in different florescences have the seven detected flavonoids such as rutin,luteolin,quercetin,isoquercitrin,trifolirhizin,kurarinone,and kushenol I. The total contents of detected alkaloids are as follows: flower buds 495. 2 μg·g-1,primal flowers 313. 7 μg·g-1,faded flowers 224. 2 μg·g-1,full bloomed flowers 193. 0 μg·g-1. The content of luteolinis relatively higher than other detected flavonoids,accounting for about 89%-94% of the total amount of detected flavonoids. The results indicated that the flower of S. flavescens could be an important material resource to obtain the resourceful alkaloids. This result can provide scientific basis for resource-oriented utilization and industrial development of the flower of S. flavescens.

关键词(KeyWords): 苦参花;花期;生物碱;黄酮;资源化利用
flower of Sophora flavescens;florescences;alkaloids;flavonoids;resources utilization

Abstract:

Keywords:

基金项目(Foundation): 江苏高校中药资源产业化过程协同创新中心建设专项;; 江苏省“333高层次人才工程”第一层次培养对象项目(2013);; 江苏省高校中药学优势学科Ⅱ期建设项目(2014-ysxk)

作者(Author): 张黄琴;朱振华;钱大玮;翁泽斌;郭盛;段金廒;雷振宏;李安平;
ZHANG Huang-qin;ZHU Zhen-hua;QIAN Da-wei;WENG Ze-bin;GUO Sheng;DUAN Jin-ao;LEI Zhen-hong;LI An-ping;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,State Administration of Traditional Chinese Medicine Key Laboratory of Chinese Medicinal Resources Recycling Utilization,National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Nanjing University of Chinese Medicine;Shanxi Zhen Dong

Email:

DOI:

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享