四物汤对辐射致血虚证小鼠血清蛋白质表达的影响The Effects of Si-Wu-Tang on Serum Protein of Blood Deficient Mice Induced by Radiation
马增春,高月,谭洪玲,王升启
MA Zeng-chun, GAO Yue, TANG Hong-ling, WANG Sheng-qi (Institute of Radiation Medicine, Beijing 100850, China)
摘要(Abstract)：
目的 :用蛋白质组技术考察四物汤对血虚证小鼠血清蛋白的影响 ,在分子水平上探讨四物汤补血的作用机理。方法 :6 0 Coγ射线全身照射小鼠造成血虚证模型 ;二向电泳、图象分析、质谱鉴定等蛋白质组学技术测定四物汤对血虚证小鼠血清中有影响的蛋白。结果 :四物汤可使血虚证小鼠血清中 12个下调和 4个上调的蛋白质有所恢复 ,进一步质谱鉴定其中 4个蛋白质可能是DNA依赖蛋白激酶 (DNA dependentproteinkinasecatalyticsub unit,P973 13 ) ,肌细胞增强蛋白 (dystrophin ,P1153 1) ,马达蛋白 (KIF13A ,Q9EQW 7) ,肌动蛋白结合蛋白 (dys tonin ,Q9QX2 0 )。它们的功能是参与DNA双链损伤的修复 ,与AP 1结合将 6 磷酸甘露糖受体从高尔基体转运到胞浆膜上 ,将细胞骨架铆定在胞浆膜上等。结论 :四物汤可能通过对血虚证小鼠血清中蛋白的影响而促进骨髓造血、减轻辐射引起的损伤。
Objective: To study the effects of Si-Wu -T ang on serum protein of blood deficient mice by proteomics technique and study t he enriching and regulating blood mechanism of Si-Wu-Tang on mocular level. Method: The blood deficient mice was induced by using a sing le dose of 3.5 Gy radiation from a 60 Coγ source, and high resolut ion two-dimensional polyacryamide gel electrophoresis(2-DE), computer-assiste d image analysis, and mass spectrometry were used to detect regulated protein by Si-Wu-Tang. Result: 12 lower and 4 higher protein in se ra could be recovered by Si-Wu-Tang, 4 protein might be DNA-dependent protein kinase catalytic subunit, Dystrophin, KIF13A, dystonin. They play a part in DNA double-stranded break repair, recombination and modulation of transcription, t ransportation of mannose-6-phosphate receptor, etc. Conclusion: Si-Wu-Tang can regulate serum protein in blood deficient mice, resulti ng in improving hematopoiesis and lessening irradiated injury.
关键词(KeyWords)：
四物汤;辐射;血清;蛋白质组
Si-Wu-Tang; irradiation; sera; proteomi cs
基金项目(Foundation): 国家自然科学基金资助项目 ( 30 0 71 91 3);; 北京市科技项目(H0 1 0 2 1 0 2 2 0 1 1 3)
作者(Author):
马增春,高月,谭洪玲,王升启
MA Zeng-chun, GAO Yue, TANG Hong-ling, WANG Sheng-qi (Institute of Radiation Medicine, Beijing 100850, China)
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