刘艺;程梅梅;董健健;周安;
LIU Yi;CHENG Mei-mei;DONG Jian-jian;ZHOU An;The Experimental Research Center,Anhui University of Chinese Medicine;Research Institute Affiliated Hospital Neurological,Anhui University of Chinese Medicine;Key Laborory of Xin'an Medicine,Ministry of Education;

• 1:安徽中医药大学科研实验中心
• 2:安徽中医药大学神经病学研究所附属医院
• 3:新安医学教育部重点实验室

摘要(Abstract)：

为了探究复方肝豆汤醇提物中主要化学成分的肠吸收特性,该文采用大鼠外翻肠囊模型,选择肝豆汤醇提物中盐酸小檗碱、槲皮素、山柰素、大黄酸、大黄酚、芦荟大黄素6种主要成分作为考察对象,评价各成分在不同肠段的吸收特征。结果表明,肝豆汤醇提物中6种成分均可进入肠囊,在高、中、低3种不同质量浓度的肝豆汤醇提物中,盐酸小檗碱、槲皮素、山柰素3种成分在不同肠段的吸收均为线性吸收,符合零级吸收速率,且在空肠与回肠中吸收速率常数K_a随着剂量增加均增加(P<0.05),符合被动吸收;而大黄酸在空肠和回肠中的K_a随着剂量增加并无显著性差异,符合主动转运。大黄酚和芦荟大黄素2种成分在肠段中吸收均较差。提示肠囊对肝豆汤醇提物中药物成分吸收具有选择性,各成分在肠囊中的吸收特征并不完全相同。
To study the intestinal absorptive characteristics of the ethanol extracts from Gandou decoction(GDD),everted intestinal sac models were used. The six representative ingredients( berberine hydrochloride,quercetin,kaempferide,rhein,chrysophanol,and aloe emodin) of GDD,were selected as the experimental targets to investigate the absorptive characteristics of various ingredients in different intestinal sections. The results showed that all six ingredients from GDD were detected in the intestinal sac,three active ingredients( berberine hydrochloride,quercetin,kaempferide) in high,medium and low doses had linear absorption properties in the small intestine segment,consistent with zero-order absorption rate; in addition,the absorption rate constant( K_a) of three components in jejunum and ileum were increased with the increase of the concentration of GDD( P < 0. 05),consistent with passive absorption. However,the K_aof rhein in jejunum and ileum showed little difference with the increase of dosage,suggesting a possibility of active transport mechanism. Chrysophanol and aloe-emodin were poorly absorbed in the two segments,which had not been detected in the previous time. The results suggested that the components of GDD were selectively absorbed in the intestinal sac,and the absorption characteristic of the ingredients were not exactly similar.

关键词(KeyWords)： 肝豆汤醇提物;多组分;外翻肠囊法;吸收速率常数
ethanol extracts of Gandou decoction;multi-component;everted intestinal sac;K_a

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81603596);; 安徽中医药大学探索性科研项目(2016ts088)

作者(Authors): 刘艺;程梅梅;董健健;周安;
LIU Yi;CHENG Mei-mei;DONG Jian-jian;ZHOU An;The Experimental Research Center,Anhui University of Chinese Medicine;Research Institute Affiliated Hospital Neurological,Anhui University of Chinese Medicine;Key Laborory of Xin'an Medicine,Ministry of Education;

DOI: 10.19540/j.cnki.cjcmm.20170523.012

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