中国中药杂志

2003, (06)

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前列倍喜胶囊定性定量方法的研究
Qualitative and Quantitative Studies on Qianliebeixi Capsules

兰燕宇,王爱民,王永林,李勇军,何迅,刘丽娜
LAN Yan-yu, WANG Ai-min, WANG Yong-lin, LI Yong-jun, HE Xun, LIU Li-na (Department of phamarcy, guiyang medical college, Guiyang 550004, Guizhou, China)

摘要(Abstract):

目的 :建立前列倍喜胶囊定性定量方法。方法 :采用薄层色谱方法对王不留行进行鉴别 ;用高效液相色谱法对制剂中槲皮素和山柰素进行含量测定。色谱条件 :用C18柱 ,以甲醇 0 .2 %磷酸水溶液 (47∶5 3)为流动相 ,流速1.0mL·min-1,柱温 4 0℃ ,检测波长 370nm。结果 :王不留行薄层色谱斑点清晰 ,阴性对照无干扰 ;含量测定中槲皮素的平均回收率为 98.9% ,RSD =2 .2 % ;山柰素的平均回收率为 10 0 .4 % ,RSD =1.7%。结论 :薄层鉴别斑点分离度好 ,易于识别 ;HPLC法简便快速、重现性好 ,能有效的控制前列倍喜胶囊的质量。
Objective: To estabilish a method of quality control on qianliebeixi capsules. Method: To clarify semen vaccarine by TLC, to determinate the contents of quertin and kaempferol in prepations. by HPLC. Chromatographic conditions: C_ 18 column with METH-0.2%H_3PO_4(47∶53) as mobile phase; the temperature of column was 40 ℃ ,and quercetin was detected at 370 nm with flowing rate 1 ml/min. Result: the spots of semen vaccarine were clear in TLC ,no interference in negative contrast The average recovery rate of quertin was 98.9% and the RSD was 2.2% ;the average recovery rate in kaempferol was 100.4% and the RSD was 1.7%. Conclusion: The resolution of spots were perfect in TLC, the spots were easy to distinguish, The method is simple, rapid as well as reproducible and can control the quality of qianliebeixi capsules effectively.

关键词(KeyWords): 前列倍喜胶囊;定性定量;槲皮素;山柰素;高效液相色谱
Qianliebeixi capsules; quality control; quertin; kaempferol; HPLC

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作者(Author): 兰燕宇,王爱民,王永林,李勇军,何迅,刘丽娜
LAN Yan-yu, WANG Ai-min, WANG Yong-lin, LI Yong-jun, HE Xun, LIU Li-na (Department of phamarcy, guiyang medical college, Guiyang 550004, Guizhou, China)

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