中国中药杂志

2008, (22) 2675-2678

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HPLC-电化学检测法用于黄芩苷和黄芩素大鼠血浆稳定性研究
Study on stability of baicalin and baicalein in mouse plasma by HPLC-ECD

汪祺;张玉杰;李维峰;杨洁;姜昊;孙万晶;
WANG Qi,ZHANG Yu-jie,LI Wei-feng,YANG Jie,JIANG Hao,SUN Wan-jing(School of Chinese Material Medica,Beijing University of Chinese Medicine,Beijing 100102,China)

摘要(Abstract):

目的:考察黄芩苷(BG)和黄芩素(B)在缓冲液及大鼠血浆中的稳定性,建立测定二者在大鼠血浆中含量的样品处理方法,并初步探讨二者在血浆中稳定性的影响因素。方法:采用HPLC-电化学检测法,分别考察BG,B在不同条件下的稳定性及使二者稳定的条件。结果:BG,B在大鼠血浆中较在pH7.4的缓冲液中更不稳定。在pH7.4缓冲液中加入抗氧化剂可使BG和B的稳定性明显提高,剩余百分含量达到102%和100%;在大鼠血浆中加入抗氧化剂及1 mol.L-1HCl可使BG和B的稳定性明显增加,剩余百分含量可分别达到98%和103%。结论:BG和B在pH7.4缓冲液中的稳定性主要受氧化影响,BG和B在血浆中的稳定性不仅与氧化有关,还受血浆中的内源物质影响,最终确定每1 mL大鼠血浆中加入抗氧剂及100μL1 mol.L-1HCl为血浆样品的处理条件,此条件可使样品稳定。
Objective: To compare the stability of baicalin and baicalein in buffered aqueous solutions and mouse plasma in order to discuss the degradation mechanism and the method of disposing samples.Method: HPLC-ECD was used to determine baicalin and baicalein in different conditions,and the disposing samples methods were selected to stabilize them.Result: Baicalin and baicalein in the buffered aqueous solution at pH 7.4 was more stable than that in the mouse plasma.After adding Vit C in the buffered aqueous solution,the stability of baicalin and baicalein could be improved obviously and their remained percents were 102% and 100%.The stability of baicalin and baicalein could be enhanced distinctly in the mouse plasma only when adding Vit C and 1 mol·L-1 HCl,and furthermore their remained percentage was up to 98% and 103%.Conclusion: The stability of baicalin and baicalein is related to the oxidation and enzyme degradation and it can be improved by adding Vit C and 1 mol·L-1 HCl in the solution.

关键词(KeyWords): HPLC-电化学;黄芩苷;黄芩素;血浆中稳定性
HPLC-ECD;baicalin;baicalein;stability in plasma

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作者(Author): 汪祺;张玉杰;李维峰;杨洁;姜昊;孙万晶;
WANG Qi,ZHANG Yu-jie,LI Wei-feng,YANG Jie,JIANG Hao,SUN Wan-jing(School of Chinese Material Medica,Beijing University of Chinese Medicine,Beijing 100102,China)

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