中国中药杂志

2020, v.45(01) 85-91

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基于多花黄精转录组的多糖及薯蓣皂苷生物合成路径研究
Biosynthetic pathways of Polygonatum cyrtonema polysaccharide and diosgenin based on its transcriptomic data

祝明珠;俞年军;王秋丽;周安;顾晓;韩荣春;童小慧;彭代银;
ZHU Ming-zhu;YU Nian-jun;WANG Qiu-li;ZHOU An;GU Xiao;HAN Rong-chun;TONG Xiao-hui;PENG Dai-yin;Institute of Traditional Chinese Medicine Resources Protection and Development, Anhui Academy of Chinese Medicine,Anhui University of Chinese Medicine;

摘要(Abstract):

多花黄精Polygonatum cyrtonema为百合科黄精属植物,其干燥根茎作为黄精药材来源之一,一直药食兼具,其主要活性成分为多糖和皂苷类。现有研究多集中于药材质量与药理药效学评价,对其药效物质的生物合成研究报道较少。为进一步探索多花黄精中多糖及薯蓣皂苷生物合成途径及其相关的结构基因,该研究对多花黄精叶、茎、根茎、根4个组织部位进行转录组测序分析。采用BGISEQ-500测序平台,共获得42.03 Gb数据;以Trinity软件对去除冗余后的全部读长进行组装,共获得137 233条转录本,其中68.13%的unigene在7个功能数据库(KEGG,GO,NR,NT,SwissProt,Pfam,KOG)中得到注释。通过数据挖掘,对可能参与多糖与薯蓣皂苷生物合成的转录本进行分析,阐释了多花黄精中该2类药效物质生合成的可能途径;应用实时定量PCR,将多花黄精多糖及皂苷合成基因的实际转录水平与其生物信息学数据进行比对,验证其表达情况。该实验为多花黄精次生代谢产物生物合成途径研究及其相关结构基因功能的阐明提供数据资料。
Polygonatum cyrtonema belongs to the plant family Liliaceae, and its dried rhizome is one of the sources of Chinese traditional medicine of Polygonati Rhizoma. It possesses the dual function as both medicine and food. Its main chemical components are polysaccharides and saponins. In order to understand the biosynthesis pathway of polysaccharides and diosgenin in P. cyrtonema, the corresponding transcriptomic data were obtained by extracting and sequencing the RNA of four parts of P. cyrtonema, namely, leaves, stems, rhizomes and roots. By adopting BGISEQ-500 sequencing platform, 42.03 Gb data were retrieved. Subsequently, the de novo assembly was carried out by Trinity software to obtain 137 233 transcripts, of which 68.13% of unigenes were annotated in seven databases including KEGG, GO, NR, NT, SwissProt, Pfam and KOG. Transcripts that may be involved in the biosynthesis of polysaccharides and diosgenin were analyzed by data mining. With help of qPCR, we validated expression data of four genes that were possibly involved in the biosynthesis of target metabolites. This experiment provides data for the study of biosynthetic pathways of P. cyrtonema secondary metabolites and the clarification of related structural gene functions.

关键词(KeyWords): 多花黄精;转录组;多糖;薯蓣皂苷;生物合成途径
Polygonatum cyrtonema;transcriptome;polysaccharide synthesis;diosgenin;biosynthetic pathway

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发计划项目(2017YFC1701600,2017YFC1701602);; 2017年中医药公共卫生服务补助专项(财社[2017]66号);; 安徽中医药大学校级探索性科研项目;安徽中医药大学自然重点项目(2019zrzd02);; 安徽高校科研创新平台团队项目(皖教秘科[2015]49号);; 池州市科技专项(17kz010107)

作者(Authors): 祝明珠;俞年军;王秋丽;周安;顾晓;韩荣春;童小慧;彭代银;
ZHU Ming-zhu;YU Nian-jun;WANG Qiu-li;ZHOU An;GU Xiao;HAN Rong-chun;TONG Xiao-hui;PENG Dai-yin;Institute of Traditional Chinese Medicine Resources Protection and Development, Anhui Academy of Chinese Medicine,Anhui University of Chinese Medicine;

DOI: 10.19540/j.cnki.cjcmm.20191104.109

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