中国中药杂志

2018, v.43(20) 4044-4049

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茉莉酸甲酯对颠茄托品烷类生物碱代谢及相关基因表达的影响
Effects of methyl jasmonate on metabolism of topical alkaloids and expression of relate genes in Atropa belladonna

杨怡;张翠平;刘兴;韦悦;吴能表;
YANG Yi;ZHANG Cui-ping;LIU Xing;WEI Yue;WU Neng-biao;School of Life Science,Southwest University;Key Laboratory of Eco-environments in Three Gorges Region,Ministry of Education;Yishui County First Middle School;Cotton Research Institute,Chinese Academy of Agricultural Sciences;

摘要(Abstract):

莨菪碱和东莨菪碱是颠茄中重要的托品烷类生物碱次生代谢产物,具有多方面药理价值。该研究采用土培法种植颠茄实生苗,设置不同浓度茉莉酸甲酯(MeJA)处理,分析不同浓度MeJA对颠茄莨菪碱和东莨菪碱的含量、生物碱合成的上游物质以及关键酶基因表达量的影响。结果表明,MeJA浓度为200μmol·L-1时更有利于颠茄生物碱的积累;对腐胺(Put)及其合成的关键酶活性的测定发现,Put的含量与其合成的关键酶活性的变化基本一致,都随MeJA浓度升高先增加后降低,Put含量在MeJA浓度为200μmol·L-1时达到最高;对PMT,TRⅠ,H6H表达量的检测发现,200μmol·L-1MeJA处理下叶片和根部TRⅠ,H6H基因均有较高的表达水平,PMT基因表达量无明显变化趋势。以上结果说明,MeJA对颠茄莨菪碱和东莨菪碱合成的调控主要依靠影响关键酶基因的表达量发挥效用,适宜浓度茉莉酸甲酯提高了颠茄叶片TRⅠ和根部TRⅠ与H6H表达量,从而促进莨菪碱的合成和莨菪碱向东莨菪碱的转化,提高颠茄体内莨菪碱和东莨菪碱的积累。
Hyoscyamine and scopolamine are important secondary metabolites of tropane alkaloid in Atropa belladonna with pharmacological values in many aspects. In this study,the seedlings of A. belladonna were planted by soil culture and treated with different concentrations of methyl jasmonate(Me JA). The contents of hyoscyamine and scopolamine,the upstream products in alkaloid synthesis,and the expression levels of key enzyme genes PMT,TRⅠand H6 H in secondary metabolites of A. belladonna seedlings were measured to clarify the mechanism of Me JA regulating alkaloids synthesis. The results showed that Me JA(200 μmol·L-1) treatment was more favorable for the accumulation of alkaloids. The content of putrescine was almost consistent with the change of key enzymes activities in the synthesis of putrescine,the both increased first and then decreased with the increased Me JA concentration and the content of putrescine reached the highest at 200 μmol·L-1 Me JA. Further detection of gene expression of PMT,TRⅠand H6 H in TAs synthesis pathway showed that no significant trend in PMT gene expression levels. The expression levels of TRⅠand H6 H in leaves and roots under 200 μmol·L-1 Me JA were the highest. It can be speculated that the regulation of the formation of hyoscyamine and scopolamine by Me JA mainly through affecting the expression of key enzyme genes. Appropriate concentration of Me JA increased the gene expression of TRⅠin both leaves and roots as well as H6 H in roots,promoting the accumulation of alkaloids and the conversion of hyoscyamine to scopolamine.

关键词(KeyWords): 茉莉酸甲酯;颠茄;莨菪碱;东莨菪碱;次生代谢
methyl jasmonate;Atropa belladonna;hyoscyamine;scopolamine;secondary metabolism

Abstract:

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基金项目(Foundation): 国家自然科学基金项目(30500041)

作者(Author): 杨怡;张翠平;刘兴;韦悦;吴能表;
YANG Yi;ZHANG Cui-ping;LIU Xing;WEI Yue;WU Neng-biao;School of Life Science,Southwest University;Key Laboratory of Eco-environments in Three Gorges Region,Ministry of Education;Yishui County First Middle School;Cotton Research Institute,Chinese Academy of Agricultural Sciences;

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