中国中药杂志

1999, (09) 3-5

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HPLC法测定独活中3种香豆素的含量
Determination of Columbianetin, Columbianetin Acetate and Osthol in Angelica pubescens Maxim .f. biserrata Shan et Yuan by HPLC

才谦,沙明,杨松松
Cai Qian, Sha Ming and Yang Songsong (Department of Traditional Chinese Pharmacy, Liaoning College of Traditional Chinese Medicine, Shenyang 110032)

摘要(Abstract):

目的:建立独活药材中二氢欧山芹醇、二氢欧山芹醇醋酸酯和甲氧基欧芹素的含量测定方法。方法:ODS柱(6.0mm×150mm),流动相甲醇乙腈0.025mol·L-1磷酸(32∶20∶48),检测波长320nm。结果和结论:本法可用于独活原药材的质量控制。
Objective:To develop a method for the determination of columbianetin, columbianetin acetate and osthol in Angelica pubescens f. biserrata Method:HPLC method, ODS column (6.0mm×150mm) with a mobile phase of methanol acetonitrile 0.025mol·L -1 phosphoric acid (32∶20∶48), and wave length detection at 320nm. Result and Conclusion:The method can be used for the quality control of raw medicinal materials.

关键词(KeyWords): 独活;含量测定;高效液相色谱法
Angelica pubescens f. biserrata ; determination; HPLC

Abstract:

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基金项目(Foundation): 国家“九五”攻关课题!No .96 90 3 0 2 0 1

作者(Author): 才谦,沙明,杨松松
Cai Qian, Sha Ming and Yang Songsong (Department of Traditional Chinese Pharmacy, Liaoning College of Traditional Chinese Medicine, Shenyang 110032)

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