中国中药杂志

2013, v.38(19) 3378-3381

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

LC-MS/MS测定大鼠血浆中莫诺苷血药浓度
Determination of morroniside concentration in rat plasma by high performance liquid chromatography-tandem mass spectrometry

熊山;李敬来;朱秀清;王晓英;吕圭源;张振清;
XIONG Shan;LI Jing-lai;ZHU Xiu-qing;WANG Xiao-ying;LV Gui-yuan;ZHANG Zhen-qing;Zhejiang Chinese Medical University;Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences;

摘要(Abstract):

莫诺苷是从中药山茱萸Cornus officinalis Sieb.&Zucc.中提取分离获得的环烯醚萜苷类化合物,具有神经保护等多种药理活性。本研究首次采用LC-MS/MS技术建立了大鼠血浆中莫诺苷浓度的测定方法。血浆样品采用蛋白沉淀法,以金丝桃苷作为内标,色谱柱为Inertsil C8-3色谱柱(2.1 mm×50 mm,5μm),流动相为水(含1 mmol·L-1甲酸钠)-乙腈梯度洗脱,流速0.4 mL·min-1。采用电喷雾离子源(ESI),正离子模式,多反应监测(MRM)。莫诺苷在2~5 000μg·L-1(r=0.995 7)线性关系良好,最低定量限为2μg·L-1。方法精密度、准确度、回收率和基质效应均符合生物样品测定的要求,适合大鼠血浆中莫诺苷浓度的测定。应用该方法进行莫诺苷在大鼠体内的药代动力学研究,给药剂量为20 mg·kg-1,绘制血药浓度-时间曲线,并采用DAS 2.0计算得主要药代动力学参数:AUC0-∞为(587.6±290.7)μg·min·L-1,C max为(334.2±148.0)μg·L-1,T max为(0.6±0.3)h,t1/2为(0.7±0.3)h。
Morroniside,an iridoid glycoside extracted from Cornus officinalis,has multiple pharmacological effects such as neuroprotection. This study took the lead in establishing a method for determining morroniside concentration in rat plasma by high performance liquid chromatography-tandem mass spectrometry. Plasma samples were processed with protein precipitation method,with hyperoside as the internal standard. An Inertsil C8-3 column( 2. 1 mm × 50 mm,5 μm) was adopted,with a mobile phase composed of water( containing 1 mmol·L- 1Sodium formate)-acetonitrile( gradient elution) at a flow rate of 0. 4 mL·min- 1. Electrospray ionization( ESI) was adopted in the positive ion mode for multi-reaction monitoring( MRM). Morroniside showed a good linear relationship ranging between 2-5 000 μg·L- 1( r = 0. 995 7),with the minimum limit of quantification of 2 μg·L- 1. Its precise,accuracy,recovery and matrix effect were all in line with the biological sample measurement requirements. Therefore,the method described above was proved to be suitable for the determination of morroniside concentration in rat plasma. To use the method in the pharmacokinetic study on morroniside in rats,oral administration dose shall be set at 20 mg·kg- 1to map the plasma concentration-time curve. Main pharmacokinetic parameters were calculated by DAS 2. 0. Specifically,AUC0-∞was( 587. 6 ± 290. 7) μg · min · L- 1,C max was( 334. 2 ±148. 0) μg·L- 1,T max was( 0. 6 ± 0. 3) h,t1 /2was( 0. 7 ± 0. 3) h.

关键词(KeyWords): 莫诺苷;LC-MS/MS;药代动力学
morroniside;LC-MS/MS;pharmacokinetics

Abstract:

Keywords:

基金项目(Foundation): 国家“重大新药创制”科技重大专项(2012ZX09301003-001-007)

作者(Author): 熊山;李敬来;朱秀清;王晓英;吕圭源;张振清;
XIONG Shan;LI Jing-lai;ZHU Xiu-qing;WANG Xiao-ying;LV Gui-yuan;ZHANG Zhen-qing;Zhejiang Chinese Medical University;Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences;

Email:

DOI:

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享