中国中药杂志

2019, v.44(06) 1151-1155

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

高卢蜜环菌几丁质酶基因的生物信息学分析与表达
Bioinformatics analysis and expression of chitinase genes in Armillaria gallica

黄靖雯;刘娟;周骏辉;袁媛;杨全;黄璐琦;
HUANG Jing-wen;LIU Juan;ZHOU Jun-hui;YUAN Yuan;YANG Quan;HUANG Lu-qi;School of Traditional Chinese Medicine,Guangdong Pharmaceutical University;National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;

摘要(Abstract):

高卢蜜环菌是一种兼性寄生真菌,在天麻栽培过程中作为天麻的唯一营养源。几丁质酶作为一种糖苷水解酶,在高卢蜜环菌的生长发育及其与天麻的共生过程中发挥重要作用。高卢蜜环菌几丁质酶基因共有22条,对这些几丁质酶基因的氨基酸序列进行生物信息分析,发现有12条几丁质酶基因均含有糖苷水解酶18家族(GH18)结构域。对高卢蜜环菌、奥氏蜜环菌、天麻、酿酒酵母和哈茨木霉的几丁质酶氨基酸序列进行系统进化分析,进一步预测高卢蜜环菌中几丁质酶基因功能。利用独角金内酯类似物GR24诱导高卢蜜环菌分枝,并分析22条几丁质酶基因的表达模式,发现8条基因可能参与蜜环菌分枝、细胞壁降解和重塑等生理过程。
Armillaria gallica is a facultative parasitic fungus which is the only nutrient source of Gastrodia elata during its cultivation.Chitinase,as a glycosidic hydrolytic enzyme,plays an important role in the growth,development,stress tolerance and symbiotic signal transduction of A. gallica. There were 22 chitinase genes in A. gallica. Bioinformatics analysis of amino acid sequence of these chitinase genes revealed that 12 chitinase genes contained glycosidase 18 family( GH18) domain. Chitinase amino acid sequences of A. gallica,A. ostoyae,G. elata,Saccharomyces cerevisiae and Trichoderma harzianum were analyzed byclustering trees,so as to further predict the gene function of chitinase in A. gallica. Induction of A. gallica branching with strigolactone analogue GR24,high-throughput sequencing technology based on the induction of branch group( MHJ1),uninduced branch group( MHJ2) and blank control group( MHJ3) is used to detect the expression quantity,the transcription level data of 22 chitinase genes were obtained and the heat map was generated for expression pattern analysis. It was found that 8 genes may be involved in physiological processes such as A. gallica branching,cell wall degradation and remodeling. In this paper,the function of chitinase gene in A. gallica was just preliminarily analyzed and predicted.

关键词(KeyWords): 蜜环菌;几丁质酶;独角金内酯;生物信息学分析;表达分析
Armillaria gallica;chitinase;strigolactone;bioinformatics analysis;expression analysis

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金重大项目(81891013,81891010);; 中央级公益性科研院所基本科研业务费专项(ZZ10-008);; 中央本级重大增减支项目(2060302)

作者(Author): 黄靖雯;刘娟;周骏辉;袁媛;杨全;黄璐琦;
HUANG Jing-wen;LIU Juan;ZHOU Jun-hui;YUAN Yuan;YANG Quan;HUANG Lu-qi;School of Traditional Chinese Medicine,Guangdong Pharmaceutical University;National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;

Email:

DOI:

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享