中国中药杂志

2014, v.39(04) 674-678

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逍遥散超临界CO_2提取工艺及提取物GC-MS特征图谱初步研究
Study on supercritical CO_2 extraction of Xiaoyaosan and its GC-MS fingerprint

左亚妹;田俊生;郭晓擎;周玉枝;高晓霞;秦雪梅;
ZUO Ya-mei;TIAN Jun-sheng;GUO Xiao-qing;ZHOU Yu-zhi;GAO Xiao-xia;QIN Xue-mei;Modern Research Center for Traditional Chinese Medicine,Shanxi University;College of Chemistry and Chemical Engineering,Shanxi University;

摘要(Abstract):

该文为了初步确定逍遥散超临界CO_2提取的最佳工艺条件,并建立提取物的气相色谱-质谱(GC-MS)特征图谱,以提取物得率为考察指标,采用正交试验设计方法,对提取压力、提取温度、CO_2流量、提取时间等因素进行考察;采用GC-MS对不同批次的提取物进行特征图谱分析。逍遥散超临界CO_2提取的最佳工艺条件为提取压力20 MPa,提取温度50℃,CO_2流量25 kg·h~(-1),提取时间3 h,提取物平均得率2.2%。GC-MS特征图谱中确定27个共有峰,占总峰面积的81.89%,其中指认出21个化合物,占提取物总量的53.20%。超临界CO_2萃取工艺合理可行,GC-MS方法准确可靠,重复性好,可用于逍遥散超临界CO_2提取物的制备及质量控制。
To determine the optimum conditions of supercritical CO_2 extraction of Xiaoyaosan,and establish its fingerprint by gas chromatography-mass spectrometry(GC-MS),the yield of extract were investigated,an orthogonal test was used to quantify the effects of extraction temperature,pressure,CO_2 flow rate and time,and fingerprint analysis of different batches of extracts were by GCMS.The optimal extraction conditions were determined as follows:extraction pressure 20 MPa,extraction temperature 50 ℃,CO_2 flow rate 25 kg·h~(-1),extraction time 3 h,and average yield 2.2%.The GC-MS fingerprint was established and 27 common peaks were found,whose contents add up to 81.89%of the total peak area.Among them,21 compounds were identified,accounting for 53.20%of the total extract The extraction process is reasonable and favorable for industrial production.The GC-MS method is accurate,reliable,reproducible,and can be used for quality control of supercritical CO_2 extract from Xiaoyaosan.

关键词(KeyWords): 逍遥散;低极性组分;超临界CO_2提取;气相色谱-质谱;特征图谱
Xiaoyaosan;low polar components;supercritical CO_2 extraction;gas chromatography-mass spectrometry;fingerprint

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81102833,81173366);; 国家国际科技合作计划项目(2011DFA32630);; 国家“重大新药创制”科技重大专项(2012ZX09103201-035);; 山西省科技攻关项目(20130313015-1)

作者(Author): 左亚妹;田俊生;郭晓擎;周玉枝;高晓霞;秦雪梅;
ZUO Ya-mei;TIAN Jun-sheng;GUO Xiao-qing;ZHOU Yu-zhi;GAO Xiao-xia;QIN Xue-mei;Modern Research Center for Traditional Chinese Medicine,Shanxi University;College of Chemistry and Chemical Engineering,Shanxi University;

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