中国中药杂志

2010, v.35(05) 616-619

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高效液相色谱同时测定夏枯草药材中4种活性成分的含量
Simultaneous determination of four active components in Spica Prunellae by HPLC

方罗;林能明;吴永江;
FANG Luo1,2,LIN Nengming2,WU Yongjiang1*(1.College of Pharmaceutical Sciences,Zhejiang University,Hangzhou 310058,China;2.Zhejiang Cancer Hospital,Hangzhou 310022,China)

摘要(Abstract):

目的:建立同时测定夏枯草药材中咖啡酸、迷迭香酸、齐墩果酸及熊果酸含量的高效液相色谱方法。方法:夏枯草样品以75%乙醇溶液(含1%甲酸)超声提取,分析色谱柱为Elite SinoChrom ODS-AP柱(4.6 mm×250 mm,5μm),以0.01%磷酸溶液-乙腈为流动相进行梯度洗脱;采用波长切换方式检测,330 nm(0~33 min),203 nm(33~40 min);柱温20℃,进样量50μL。结果:咖啡酸、迷迭香酸、齐墩果酸与熊果酸的峰面积与其质量浓度之间均具有良好的线性关系,平均加样回收率93.7%~105.2%,RSD值均小于4.5%,所测夏枯草样品中咖啡酸、迷迭香酸、齐墩果酸与熊果酸的质量分数分别为0.040 1~0.096 8,0.99~2.57,0.243~0.556,4.06~8.13 mg.g-1。结论:本方法简便、快速、准确,可同时测定夏枯草中咖啡酸、迷迭香酸、齐墩果酸和熊果酸的含量。
Objective: To develop a HPLC method for simultaneous determination of caffeic acid,rosmarinic acid,oleanolic acid and ursolic acid in Spica Prunellae.Method: After ultrasonic extraction with 75% ethanol solution containing 1% formic acid,the ethanol-extract of Spica Prunellae was analyzed on an Elite SinoChrom ODS-AP column using gradient elution of 0.01% phosphoric acid(A) and acetonitrile(B) at a flow rate of 0.9-1.0 mL·min-1.A wavelength switch program was used for detection at 330 nm(0-33 min) and 203 nm(33-40 min).The column temperature was set at 20 ℃ and injection volume was 50 μL.Result: The calibration curves of all analytes were linear.The average recoveries were 93.7%-105.2% with RSDs not more than 4.5%.The contents of caffeic acid,rosmarinci acid,ursolic acid and oleanolic acid in Spica Prunellae were 0.0401-0.0968,0.99-2.57,0.243-0.556,4.06-8.13 mg·g-1,respectively.Conclusion: The described method is sensitive,convenient and accurate,and is suitable for the simultaneous determination of caffeic acid,rosmarinic acid,oleanolic acid and ursolic acid in Spica Prunellae.

关键词(KeyWords): 夏枯草;咖啡酸;迷迭香酸;齐墩果酸;熊果酸;高效液相色谱法
Spica Prunellae;caffeic acid;rosmarinic acid;oleanolic acid;ursolic acid;HPLC

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作者(Author): 方罗;林能明;吴永江;
FANG Luo1,2,LIN Nengming2,WU Yongjiang1*(1.College of Pharmaceutical Sciences,Zhejiang University,Hangzhou 310058,China;2.Zhejiang Cancer Hospital,Hangzhou 310022,China)

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