赖宏武;齐耀东;刘海涛;刘久石;张本刚;
LAI Hong-wu;QI Yao-dong;LIU Hai-tao;LIU Jiu-shi;ZHANG Ben-gang;Key Laboratory of Bioactive Substance and Resources Utilization of Chinese Herbal Medicine,Ministry of Education,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Peking Union Medical College;

• 1:中国医学科学院北京协和医学院药用植物研究所中草药物质基础与资源利用教育部重点实验室

摘要(Abstract)：

湖北贝母为传统中药,然而《Flora of China》将其基原植物湖北贝母Fritillaria hupehensis归并于天目贝母F.monantha项下。该实验采用分子系统学方法,以川百合Lilium davidii为外类群,用核基因ITS序列和叶绿体基因rpl16序列、matK序列等3个片段对湖北贝母及其近缘类群天目贝母F.monantha、安徽贝母F.anhuiensis等进行联合建树分析,对湖北贝母植物的系统位置进行了探讨,为湖北贝母药材的安全使用提供分子证据。结果显示,分子系统树上,3种贝母各自的居群聚为一支,之后天目贝母与安徽贝母聚为一支,最后与湖北贝母聚为一支。表明湖北贝母与天目贝母的亲缘关系可能要远于安徽贝母与天目贝母之间的关系,因此不适宜将湖北贝母归并于天目贝母。
The systematic position of Fritillaria hupehensis has been in dispute.Phylogentic analyses were conducted on sequences of ITS,rpl16,matK sequences for species of F.hupehensis and allies.Lilium davidii was designed as outgroup.The analyses were performed using MP and ML methods.Conclusions could be achieved as follow.The topologies of MP and ML are consistent.The samples of F.hepehensis from different places form a supported clade with a strong bootstrap.And then form a strongly supported clade with F.anhuiensis,F.monantha.The results suggests that although F.hupehensis has a closet relation with the two ones,it exists some difference.

关键词(KeyWords)： 湖北贝母;天目贝母;安徽贝母;ITS;rpl16;matK
Fritillaria hupehensis;Fritillaria monantha;Fritillaria anhuiensis;ITS;rpl16;matK

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 赖宏武;齐耀东;刘海涛;刘久石;张本刚;
LAI Hong-wu;QI Yao-dong;LIU Hai-tao;LIU Jiu-shi;ZHANG Ben-gang;Key Laboratory of Bioactive Substance and Resources Utilization of Chinese Herbal Medicine,Ministry of Education,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Peking Union Medical College;

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