中国中药杂志

2008, (18) 2116-2119

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

人参皂苷Rh_2对小鼠移植瘤生长及对细胞间连接黏附分子表达的影响
Effect of ginsenoside Rh_2 on transplanted-tumor and expression of JAM in mice

王强;吴美清;赵玲辉;杨慧科;吕晓红;
WANG Qiang1,WU Mei-qing1,ZHAO Ling-hui2,YANG Hui-ke2,LV Xiao-hong2 (1.Inner Mongolia Forestry General Hospital,Yakeshi 022150,China;2.Department of Anatomy,Harbin Medical University,Harbin 150081,China)

摘要(Abstract):

目的:观察人参皂苷Rh2对小鼠移植瘤生长及其对细胞间连接黏附分子(JAM)在癌组织表达的影响,探讨其抗肿瘤作用。方法:昆明种小鼠40只,前肢皮下接种S180小鼠癌性腹水0.2 mL,成瘤后随机分为2组,实验组给予人参皂苷Rh2(20 mg.kg-1)灌服,2 mL/只;对照组给予等量生理盐水,6周后取材。用免疫组化法观察JAM-1,JAM-2在癌细胞、淋巴管及血管的表达。结果:对照组JAM-1阳性表达的肿瘤细胞吸光度(A)549.90,实验组340.55,P<0.05;JAM-2阳性表达弱。对照组JAM-1和JAM-2阳性表达的血管数密度分别为2.33和1.34,实验组分别为1.09和0.9。对照组JAM-1和JAM-2阳性表达的淋巴管数密度分别为2.23和1.88,实验组分别为0.99和0.79;两组间的差异均为P<0.05。结论:人参皂苷Rh2通过下调JAM在肿瘤细胞的表达,抑制肿瘤组织血管及淋巴管的生成,进而抑制肿瘤生长。
Objective: To discuss the anti-tumor activity of ginsenoside Rh2,we observed the expressions of the junction adhesion molecul(JAM) in transplanted-tumor in mice.Method: The models of 40 transplanted-tumor mice that were established by subsequently injecting cancer ascite of mice(S180) with 0.2 mL per mouse into the preepipodite skin were divided into two groups.Experiment group was drenched with 2 mL ginsenoside Rh2 per mouse,equating to a dose 20 mg·kg-1.Control group was drenched with 2 mL normal saline per mouse.The expression of JAM-1,JAM-2 in the lymphatics,blood vessels and tumours were observed by immunohistochemical staining.Result: The expression of JAM-1 on the cancer cells was significantly decreased in experiment group(IA 340.55) as compared with control group(IA 549.90,P<0.05).However,JAM-2 weakly expressed in both two groups.The density of blood vessels in which JAM-1,JAM-2 expressed showed 2.33 and 1.34 in control group,and 1.09 and 0.9 in experiment group respectively.Moreove,the density of lymph vessels were respectively 2.23 and 1.88 in control group compared with 0.99 and 0.79 in experiment group.The expression in blood vessels and lymph vessels in control group were significantly higher than those in experiment group,respectively(P<0.05).Conclusion: Ginsenoside Rh2 can affect the tumor growth,further angiogenesis and lymphangiogenesis by down-regulating JAM expression in tumor.

关键词(KeyWords): 人参皂苷Rh2;移植瘤;细胞间连接黏附分子
ginsenoside Rh2;transplanted-tumor;JAM

Abstract:

Keywords:

基金项目(Foundation): 黑龙江省科技计划项目(GC03C606-1)

作者(Author): 王强;吴美清;赵玲辉;杨慧科;吕晓红;
WANG Qiang1,WU Mei-qing1,ZHAO Ling-hui2,YANG Hui-ke2,LV Xiao-hong2 (1.Inner Mongolia Forestry General Hospital,Yakeshi 022150,China;2.Department of Anatomy,Harbin Medical University,Harbin 150081,China)

Email:

DOI:

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享