中国中药杂志

2016, v.41(04) 705-710

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茅苍术及其麸炒品对胃溃疡大鼠抗炎作用的比较研究
Comparison of anti-inflammatory activity between crude Atractylodes lancea and their processed products by stir-baking with bran in rat models of gastric ulcer

于艳;贾天柱;才谦;
YU Yan;JIA Tian-zhu;CAI Qian;Experimental Center of Traditional Chinese Medicine,the Affiliated Hospital,Liaoning University of Traditional Chinese Medicine;School of Pharmacy,Liaoning University of Traditional Chinese Medicine;

摘要(Abstract):

该实验旨在比较茅苍术生品和麸炒品对实验性胃溃疡大鼠的抗炎作用,初步探讨茅苍术抗胃溃疡的作用机制。采用改良Okabe法即外科手术胃黏膜局部注射乙酸法诱导大鼠胃溃疡模型。大鼠分成10组,即假手术组,模型组,奥美拉唑组,三九胃泰组,茅苍术生品和麸炒品低、中、高剂量组,灌胃给药,每日2次,连续给药10 d,腹主动脉采血,分离血清,分取胃组织溃疡面。采用酶联免疫吸附(ELISA)法检测血清及胃组织中炎症因子白细胞介素6(IL-6)、白细胞介素8(IL-8)、肿瘤坏死因子α(TNF-α)和前列腺素E2(PGE2)的含量。实时荧光定量聚合酶链式反应(RT-PCR)法检测胃组织中IL-8和TNF-αmRNA的表达。免疫组织化学法检测胃组织中TNF-α和IL-8的蛋白表达。与假手术组比较,模型组大鼠血清及胃组织中IL-6,IL-8,TNF-α和PGE2的含量明显升高,胃组织中IL-8和TNF-αmRNA的表达和二者的蛋白表达量显著增加,各治疗组均出现不同程度的降低。与茅苍术生品比较,相同剂量的茅苍术麸炒品具有更好的降低血清及胃组织中IL-6,IL-8,TNF-α和PGE2的含量,下调胃组织中IL-8和TNF-αmRNA表达的作用,其中高剂量组及中剂量组均有显著性差异。茅苍术对乙酸致胃溃疡大鼠具有明显的抗炎作用,麸炒后该作用增强;茅苍术抗胃溃疡的作用部分是由于下调IL-6,IL-8,TNF-α和PGE2的含量。
To compare the anti-inflammatory activity of the crude Atractylodes lancea( AL) and AL processed products by stir-baking with bran in rat models of gastric ulcer,and preliminarily explore the anti-ulcer mechanisms of AL,the model of gastric ulcer was imitated by local acetic acid injection into gastric mucosa in rats by surgery according to the modified Okabe method. All rats were randomly divided into the following 10 groups: sham-operation group,model group,omeprazole group,Sanjiu Weitai granule group,crude AL low dose group,crude AL middle dose group,crude AL high dose group,processed AL low dose group,processed AL middle dose group,and processed AL high dose group. Rats were administered via intragastric( ig) two times each day,for 10 consecutive days.Blood was collected from the abdominal aorta,serum was separated,and the ulcer tissues were taken. The levels of inflammatory factors interleukin 6,8( IL-6,8),tumor necrosis factor-α( TNF-α),and prostaglandin E2( PGE2) in serum and gastric tissues were determined by enzyme-linked immunosorbent assay( ELISA),and the mRNA expressions of TNF-α and IL-8 in gastric tissues were detected by quantitative real-time reverse transcription polymerase chain reaction( RT-PCR). The protein expressions of TNF-α and IL-8in gastric tissues were detected by immunohistochemistry. Compared with sham-operation group,the levels of TNF-α,IL-8,IL-6,PGE2 as well as the mRNA expressions and protein expressions of TNF-α,IL-8 in gastric tissues were significantly higher in model group. The above levels were reduced in different degrees in all treatment groups. Compared with the crude AL,same dose of processed AL was more effective in decreasing the levels of TNF-α,IL-8,IL-6,PGE2 in serum and gastric tissues and down-regulating the mRNA expressions of TNF-α and IL-8 in gastric tissues,with significant difference in middle dose groups and high dose groups. The results showed that AL had potent anti-inflammatory effects in rat models of gastric ulcer induced by acetic acid,and the processed AL had more obvious effect. The anti-ulcer action of AL could be attributed partly to down-regulating the levels of TNF-α,IL-8,IL-6 and PGE2.

关键词(KeyWords): 茅苍术;胃溃疡;炮制;ELISA;RT-PCR;免疫组织化学
Atractylodes lancea(AL);gastric ulcer;processing;ELISA;RT-PCR;immunohistochemistry

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81202919);; 国家中医药管理局中医药行业科研专项(20110700712)

作者(Authors): 于艳;贾天柱;才谦;
YU Yan;JIA Tian-zhu;CAI Qian;Experimental Center of Traditional Chinese Medicine,the Affiliated Hospital,Liaoning University of Traditional Chinese Medicine;School of Pharmacy,Liaoning University of Traditional Chinese Medicine;

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