中国中药杂志

2017, v.42(11) 2072-2077

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宁夏枸杞内生菌的抗菌和抗肿瘤活性研究
Antimicrobial and anti-tumor activities of endophytes from Lycium barbarum of Ningxia

代金霞;杜晓宁;
DAI Jin-xia;DU Xiao-ning;School of Life Science,Ningxia University;

摘要(Abstract):

为筛选具有抗菌抗肿瘤活性的枸杞内生菌资源,对分离自宁夏枸杞的10株内生菌发酵液的抑菌活性和体外细胞毒活性进行检测,通过ITS和16S rDNA序列分析对菌株进行了分子鉴定。结果表明,5株内生真菌对供试病原菌都未表现出抑制活性,放线菌AL6对3种病原真菌都有一定的抑制作用,而AL5仅对金黄色葡萄球菌有较强抑制活性。但内生真菌的抗肿瘤活性明显强于放线菌。当发酵液浓度为0.2 g·L~(-1)时,有4株内生真菌至少对1种肿瘤细胞的抑制率高于50%。序列分析表明,5株内生真菌隶属于曲霉属、青霉属和翘孢霉属。5株内生放线菌隶属于链霉菌属、微球菌属和拟诺卡氏菌属。曲霉属菌株FL1对A549和HeLa细胞的抑制活性都较强,其IC_(50)分别为0.022,0.028 g·L~(-1),值得进一步研究。
The antimicrobial activity and cytotoxicity in vitro of the fermentation broth of 10 endophytic strains isolated from Lycium barbarum were determined to screen high activity endophytic strains. Sequences analysis of ITS and 16S rDNA was used for molecular identification of the strains. The results showed that 5 endophytic fungi had no inhibitory activity against the tested pathogens. Endophytic actinomycete strain AL6 had a certain inhibitory effect on 3 kinds of pathogenic fungi,and strain AL5 only had strong inhibitory activity against Staphylococcus aureus. However,the anti-tumor activity of endophytic fungi was significantly higher than that of actinomycetes. Four endophytic fungi strains exhibited the growth inhibition rate of above 50% against at least one of the tested tumor cells when the concentration of fermentation broth was 0. 2 g·L~(-1). Sequences analysis showed that 5 endophytic fungi strains belonged to genus Aspergillus,Penicillium and Emericella,and the 5 endophytic actinomycetes strains belonged to genus Aspergillus,Penicillium and Emericella. Aspergillus strain FL1 had stronger inhibitory activity against A549 and HeLa cells,and the IC_(50) values were 0. 022,0. 028 g·L~(-1),respectively,which was worthy of further study.

关键词(KeyWords): 枸杞;内生菌;抑菌活性;抗肿瘤活性
Lycium barbarum;endophytes;anti-microbial activities;anti-tumor activities

Abstract:

Keywords:

基金项目(Foundation): 宁夏自然科学基金项目(NZ1601)

作者(Author): 代金霞;杜晓宁;
DAI Jin-xia;DU Xiao-ning;School of Life Science,Ningxia University;

Email:

DOI: 10.19540/j.cnki.cjcmm.20170415.001

参考文献(References):

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