曾品涛;周慧;郑一敏;胥秀英;傅善权;
ZENG Pintao,ZHOU Hui,ZHENG Yimin,XU Xiuying,FU Shanquan(School of Pharmacy and Bioengineering,Chongqing University of Technology,Chongqing 400050,China)

• 1:重庆理工大学药学与生物工程学院

摘要(Abstract)：

采用反相高效液相色谱法同时测定麦冬中6-甲酰基沿阶草酮甲、甲基沿阶草酮甲和沿阶草酮甲的含量,选用乙腈-0.5%磷酸溶液(58∶42)作为流动相,检测波长为296 nm(0～14 min),275 nm(14～22 min)。6-甲酰基沿阶草酮甲、甲基沿阶草酮甲和沿阶草酮甲检测的线性范围分别为0.165～0.990(r=0.999 9),0.153～0.918(r=0.999 9),0.270～1.620μg(r=0.999 9),平均加样回收率分别为99.76%(RSD 0.95%),99.86%(RSD 0.82%),99.41%(RSD1.1%)。研究所建立的反相高效液相色谱法法简便快速、准确度高,可用于麦冬药材的质量控制。
Three homoisoflavonoids,6-aldehydo-3-ophiopogonanone A,methyl ophiopogonanone A and ophiopogonanone A from Ophiopogon japonicus were analyzed simultaneously by HPLC with acetonitrile-water containing 0.5% H3PO4(58∶42) as the mobile phase,and the detection wavelength was set at 296 nm(0-14 min) and 275 nm(14-22 min).The mean recoveries of three homoisoflavonoids were 99.41%-99.86%(RSD 0.82%-1.05%).The linear response ranges of.6-aldehydo-3-ophiopogonanone A,methyl ophiopogonanone A and ophiopogonanone A were 0.165-0.990 μg(r=0.999 9),0.153-0.918 μg(r=0.999 9),and 0.270-1.620 μg(r=0.999 9),respectively.This method was certified to be accurate and reliable and can be used for quality control of O.japonicus.

关键词(KeyWords)： 麦冬;高效液相色谱;6-甲酰基沿阶草酮甲;甲基沿阶草酮甲;沿阶草酮甲;含量测定
Ophiopogonis Radix;6-aldehydo-3-ophiopogonanone A;methyl ophiopogonanone A;ophiopogonanone A;HPLC;quantitative determination

Abstract:

Keywords:

基金项目(Foundation): 国家“重大新药创制”科技重大专项(2010ZX09401-306-2-23);; 重庆市科委产业化项目(CSTC,2008AB5116)

作者(Author): 曾品涛;周慧;郑一敏;胥秀英;傅善权;
ZENG Pintao,ZHOU Hui,ZHENG Yimin,XU Xiuying,FU Shanquan(School of Pharmacy and Bioengineering,Chongqing University of Technology,Chongqing 400050,China)

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DOI:

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