中国中药杂志

2011, v.36(16) 2157-2161

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PEG胁迫对黄芩黄酮类有效成分积累及相关基因表达的影响
Effects of PEG stress on flavonoids accumulation and related gene expression in suspension of Scutellaria baicalensis

杨兆春;袁媛;陈敏;帅凌飞;肖倩;林淑芳;
YANG Zhaochun,YUAN Yuan,CHEN Min,SHUAI Lingfei,XIAO Qian,LIN Shufang(Institute of Chinese Materia Medica,China Academy of Chinese Medicinal Sciences,Beijing 100700,China)

摘要(Abstract):

目的:研究不同程度PEG胁迫对黄芩悬浮细胞中黄芩苷、黄芩素的积累及相关基因表达的影响。方法:采用HPLC法检测黄芩悬浮细胞中黄芩苷、黄芩素的含量,用酶标仪检测细胞内游离脯氨酸含量,并用半定量RT-PCR的方法对相关基因的表达进行分析。结果与结论:黄芩悬浮细胞内游离脯氨酸含量随PEG胁迫浓度升高而升高,10%PEG胁迫黄芩悬浮细胞可以模拟干旱条件,提高悬浮细胞PAL基因表达量且会促进黄芩素的积累。20%PEG胁迫提高了UBGAT基因的表达量,但同时APX酶活性的提高抑制了黄芩素参与清除活性氧,导致了黄芩素向黄芩苷的转化。
Objective: To study the effects of PEG stress on baicalin,baicalein accumulation induced by an increased concentration of PEG solution and the related genes′ expression in suspension of Scutellaria baicalensis.Method: The content of baicalin,baicalein in suspension of S.baicalensis was determined by HPLC.The related genes′ expression was analyzed by semi-quantitative PCR.Result: The content of proline in suspension of S.baicalensis was promoted by PEG treatment.Ten percent PEG treatment promoted the expression of PAL and the content of baicalein in experimental material via a drought stress.20%PEG solution treatment promoted the expression of UBGAT.At the same time,the increased activity of APX inhibited the progress of eliminating reactive oxygen by baicalein,which induced the transformation from baicalein to baicalin.Conclusion: Active ingredient in suspension of S.baicalensis was promoted significantly via a stress of light concentration of PEG solution.

关键词(KeyWords): 黄芩;悬浮细胞;PEG;半定量RT-PCR;基因表达;黄芩苷;黄芩素
Scutellaria baicalensis;suspension cells;PEG;semi-quantitative PCR;gene expression;baicalin;baicalein

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基金项目(Foundation): 国家科技重大专项(2009ZX09301-005-03);; 北京市科技新星计划项目(2008B2);; 中国博士后科学基金特别资助项目(201003228)

作者(Author): 杨兆春;袁媛;陈敏;帅凌飞;肖倩;林淑芳;
YANG Zhaochun,YUAN Yuan,CHEN Min,SHUAI Lingfei,XIAO Qian,LIN Shufang(Institute of Chinese Materia Medica,China Academy of Chinese Medicinal Sciences,Beijing 100700,China)

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