秦双双;黄璐琦;袁媛;余丽莹;
QIN Shuang-shuang;HUANG Lu-qi;YUAN Yuan;YU Li-ying;Guangxi Botanical Garden of Medicial Plants;State Key Laboratory of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;

• 1:广西壮族自治区药用植物园
• 2:中国中医科学院中药资源中心道地药材国家重点实验室培育基地

摘要(Abstract)：

通过对华南忍冬Lonicera confusa绿原酸、木犀草素生物合成关键酶基因进行组织特异性表达分析,阐明华南忍冬活性成分形成的分子调控机制。采用实时定量PCR分析方法分析绿原酸和木犀草素生物合成途径上的PAL,4CL,C4H,CHS,CHI,FNS,HQT基因家族成员在华南忍冬花蕾和叶中的表达情况。结果表明PAL1,C4H1,4CL1,CHS1,CHI3,HQT2在华南忍冬花蕾中的表达水平要低于叶,而PAL3,4CL2,CHI2,FNS2在花蕾中的表达水平要高于叶。推测华南忍冬PAL3,4CL2基因可能与绿原酸成分积累有关,同时PAL1,CHS1,CHI3,HQT2同源基因在华南忍冬花蕾和叶中的表达模式与忍冬不同,这为进一步研究忍冬与华南忍冬活性成分差异的遗传机制提供理论依据。
This study analysed the tissue specific expression of critical genes involved in chlorogenic acid and luteolin biosynthesis,for exploiting the molecular mechanism of components biosynthesis in Lonicera confusa. Expression of PAL,4CL,C4H,CHS,CHI,FNS and HQT gene families of chlorogenic acid and luteolin biosynthesis-related genes in buds and leaves of L. confusa were analyed by Real-time PCR. Expressions of PAL1,C4H1,4CL1,CHS1,CHI3 and HQT2 in buds were lower than that in leaves,and expressions of PAL3,4CL2,CHI2 and FNS2 in buds were higher than that in leaves. The results indicated that that PAL3 and 4CL2 may be associated with accumulation of chlorogenic acid,and the expression patterns of PAL1,CHS1,CHI3 and HQT2 in buds and leaves of L. confusa were different with L. japonica. This study provided some theoretical basis for the further research on genetic mechanism of active components differences in L. confusa and L. japonica.

关键词(KeyWords)： 华南忍冬;绿原酸;木犀草素;关键酶基因
Lonicera confusa;chlorogenic acids;luteolin;critical genes

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81373959,81001605);; 中医药行业科研专项(201407003)

作者(Author): 秦双双;黄璐琦;袁媛;余丽莹;
QIN Shuang-shuang;HUANG Lu-qi;YUAN Yuan;YU Li-ying;Guangxi Botanical Garden of Medicial Plants;State Key Laboratory of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;

Email:

DOI:

参考文献(References)：

• [1]梁远园,卢瑞琦,张言,等.华南忍冬藤HPLC指纹图谱研究[J].中国实验方剂学杂志,2013,19(10):138.
• [2]郑亦文,马东来.木犀草素对大鼠腹腔巨噬细胞释放H2O2的影响[J].中国药理学通报,1990,6(1):56.
• [3]Lim D,Cho H,Kim J,et al.Luteolin decreases IGF-II production and downregulates insulin-like growth factor-I receptor signaling in HT-29 human colon cancer cells[J].BMC Gastroenterol,2012,12:9.
• [4]Fraser C M,Chapple C.The phenylpropanoid pathway in Arabidopsis[J].Arabidopsis Book,2011,9:e0152.
• [5]Ververidis F,Trantas E,Douglas C,et al.Biotechnology of flavonoids and other phenylpropanoid-derived natural products.Part I:chemical diversity,impacts on plant biology and human health[J].Biotechnol J,2007,2(10):1214.
• [6]Yuan Y,Wang Z Y,Jiang C,et al.Exploiting genes and functional diversity of chlorogenic acid and luteolin biosyntheses in Lonicera japonica and their substitutes[J].Gene,2012,9:51.
• [7]Owens D K,Alerding A B,Crosby K C,et al.Functional analysis of a predicted flavonol synthase gene family in Arabidopsis[J].Plant Physiol,2008,147:1046.
• [8]Ancheol C,Myungho L,Shinwoo L,et al.Tomato phenylalanine ammonia-lyase gene family,highly redundant but strongly underutilized[J].J Biol Chem,2008,283(48):33591.
• [9]Hu W J,Kawaoka A,Tsai C J,et al.Compartmentalized expression of two structurally and functionally distinct 4-coumarate:CoA ligase genes in aspen(Populus tremuloides)[J].Proc Natl Acad Sci USA,1998,95(9):5407.
• [10]Li Y,Ogita S,Keya C A,et al.Expression of caffeine biosynthesis genes in tea(Camellia sinensis)[J].Z Naturforsch C,2008,63:267.
• [11]Rani A,Singh K,Ahuja P S,et al.Molecular regulation of catechins biosynthesis in tea[Camellia sinensis(L.)O.Kuntze][J].Gene,2012,495:205.
• [12]Roslan N D,Yusop J M,Baharum S N,et al.Flavonoid biosynthesis genes putatively identified in the aromatic plant polygonum minus via expressed sequences tag(EST)analysis[J].Int J Mol Sci,2012,13:2692.
• [13]刘涛,牟兰,梁艳丽,等.灯盏花查尔酮合成酶基因表达与灯盏乙素含量关系的研究[J].中国中药杂志,2013,38(14):2241.
• [14]Huang L,Wang H,Ye H,et al.Differential expression of benzophenone synthase and chalcone synthase in Hypericum sampsonii[J].NPC,2012,7(12):1615.
• [15]Zhao X,Ge Y,Wang D,et al.Determination of extracted chlorogenic acid and luteolin-7-O-glucoside in Flos Lonicerae by HPLCDAD[J].Chin J Anal Lab,2010,29:77.
• [16]邓玲姣,周晓舟,黄素梅,等.忻城县金银花及其茎、叶中绿原酸含量测定[J].广西农学报,2007,22(5):34.
• [17]赵成.山银花不同器官的绿原酸含量及体外抑菌效果比较[J].安徽医药,2006,10(8):584.