秦双双;黄璐琦;袁媛;余丽莹;

• 1:广西壮族自治区药用植物园
• 2:中国中医科学院中药资源中心道地药材国家重点实验室培育基地

摘要(Abstract)：

通过对华南忍冬Lonicera confusa绿原酸、木犀草素生物合成关键酶基因进行组织特异性表达分析,阐明华南忍冬活性成分形成的分子调控机制。采用实时定量PCR分析方法分析绿原酸和木犀草素生物合成途径上的PAL,4CL,C4H,CHS,CHI,FNS,HQT基因家族成员在华南忍冬花蕾和叶中的表达情况。结果表明PAL1,C4H1,4CL1,CHS1,CHI3,HQT2在华南忍冬花蕾中的表达水平要低于叶,而PAL3,4CL2,CHI2,FNS2在花蕾中的表达水平要高于叶。推测华南忍冬PAL3,4CL2基因可能与绿原酸成分积累有关,同时PAL1,CHS1,CHI3,HQT2同源基因在华南忍冬花蕾和叶中的表达模式与忍冬不同,这为进一步研究忍冬与华南忍冬活性成分差异的遗传机制提供理论依据。

关键词(KeyWords)： 华南忍冬;绿原酸;木犀草素;关键酶基因

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81373959,81001605);; 中医药行业科研专项(201407003)

作者(Author): 秦双双;黄璐琦;袁媛;余丽莹;

Email:

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