中国中药杂志

1995, (07)

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RP-HPLC法测定中药材黄柏中小檗碱的含量
RP-HPLC Determination of Berberine in Cortex Phellodendri

王玉杰,朱红,王大威,王坤
Wang Yujie;Zhu Hong;Wang Dawei and Wang Kun(Changchun Municipal Institute for Drug Control 130051)

摘要(Abstract):

用反相高效液相色谱法,安定为内标,ZorbaxC_8柱,含30mmol/L。磷酸的乙腈-水(35:65)为流动相,于紫外346nm处测定了黄柏中小檗碱的含量。排除了巴马汀对小檗碱的干扰。平均回收率为101.7%,RSD为1.83%(n=20)。
A reversedphase high performance liquid chromatographic mothed was developed for the determina-tion of berberine in Cortex Phellodendri using a bonded phase column(Zorbax C_8) with diazepam as the internal stan-dard.The column temperature was 25 C.The mobile phatse was a mixture of acetonitrile-water(35:65,containing30mmol/L H_3PO_4) with a flow rate of lml/min.Berberine was detected by UV absorption at 346nm. The standardcurve was linear in the range of 0. 14 to 2. 0 μg. The recovery was 101.7% and RSI) 1.83%.

关键词(KeyWords): RP-HPLC,黄柏,小檗碱
RP-HPLC;Cortex Phelledendri ;berberine

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作者(Author): 王玉杰,朱红,王大威,王坤
Wang Yujie;Zhu Hong;Wang Dawei and Wang Kun(Changchun Municipal Institute for Drug Control 130051)

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