中国中药杂志

2020, v.45(15) 3651-3658

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山沉香结香前后细菌群落结构与多样性分析
Analysis of bacterial community structure and diversity during mountain-agarwood formation

刘娟;高佳琪;陈苏依勒;蒋超;袁媛;焦顺刚;孟虎彪;柴兴云;黄璐琦;
LIU Juan;GAO Jia-qi;CHEN Su-yi-le;JIANG Chao;YUAN Yuan;JIAO Shun-gang;MENG Hu-biao;CHAI Xing-yun;HUANG Lu-qi;National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences;School of Pharmacy, Jiangsu University;Alashan League Mongolian Medicine Hospital;Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine;

摘要(Abstract):

山沉香为木犀科羽叶丁香去皮的根、茎和粗枝,是我国有特色的蒙古族习用药材,在心脑血管疾病使用上具有良好的临床疗效,但其形成速度极为缓慢,且栽培品中挥发油含量较低。因此,如何加快山沉香的形成速度、提高挥发油含量是亟待解决的科学问题。该文采用16S rDNA扩增子测序方法,对野生、栽培结香前后的山沉香细菌群落进行系统分析。采用物种多样性曲线及Alpha多样性分析发现,山沉香的结香部位较非结香部位具有更明显的物种多样性;采用Beta多样性分析发现,野生山沉香结香部位与其他样品细菌群落结构差距较大。同时,通过细菌群落组成和物种丰度分析发现,4组山沉香样品中共有的3类优势菌属为鞘氨醇单胞菌属Sphingomonas,Modestobacter和蓝细菌的未知属unidentified Cyanobacteria;山沉香结香材料中放线菌属Actinoplanes和微杆菌属Microbacterium为优势菌属,而玫瑰单胞菌属Roseomonas在山沉香的未结香样品中丰度较高。该文首次研究了山沉香结香前后细菌的菌落结果变化,为探索山沉香的结香机制提供基础数据,为合理利用山沉香内生细菌资源提供科学依据。
As an important substitute for agarwood, mountain-agarwood, belonging to the family Oleaceae, comes from the root, stem and thick branch of Syringa pinnatifolia, which has a wide range of application in Inner Mongolia, China. It has good clinical efficacy in the use of cardiovascular diseases. However, the formation speed of mountain-agarwood is extremely slow, and its cultivated seedlings have low resin content. Therefore, how to speed up the formation of mountain-agarwood and increase the resin content is a hot research topic in this field. In this work, 16 S rDNA amplicon sequencing method was used to systematically analyze the bacterial communities of different samples of mountain-agarwood. Our data revealed that the samples of mountain-agarwood had more obvious species diversity than the ones of non-mountain-agarwood, especially the wild mountain-agarwood samples. By analysis of bacterial community composition and species abundance, Sphingomonas, Modestobacter and unidentified Cyanobacteria genus were three dominant bacterial genera in all samples. In addition, there are two identified genera of dominant bacteria, namely Actinoplanes and Microbacterium in both wild and cultivated mountain-agarwood, by bacterial community composition and species richness analysis. Meanwhile, Roseomonas was the dominant bacterial genus in both wild and cultivated non-mountain-agarwood samples. Our work could provides basic data for exploring the mechanism of the mountain-agarwood formation, and help to exploit resource of endophytic bacteria reasonably.

关键词(KeyWords): 山沉香;细菌;种群结构;种群多样性
mountain-agarwood;bacterium;population structure;population diversity

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发计划项目(2019YFC1711000);; 中央本级重大增减支项目(2060302);; 中央级公益性科研院所基本科研业务费专项(ZZXT201904)

作者(Author): 刘娟;高佳琪;陈苏依勒;蒋超;袁媛;焦顺刚;孟虎彪;柴兴云;黄璐琦;
LIU Juan;GAO Jia-qi;CHEN Su-yi-le;JIANG Chao;YUAN Yuan;JIAO Shun-gang;MENG Hu-biao;CHAI Xing-yun;HUANG Lu-qi;National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences;School of Pharmacy, Jiangsu University;Alashan League Mongolian Medicine Hospital;Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine;

Email:

DOI: 10.19540/j.cnki.cjcmm.20200527.106

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