中国中药杂志

2010, v.35(06) 682-685

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光照对黄芩黄酮类活性成分积累及其相关基因表达的影响
Effects of light on flavonoids accumulation and related gene expression in suspension cultures of Scutellaria baicalensis

陈顺钦;袁媛;罗毓健;黄璐琦;陈平;李晓明;
CHEN Shunqin1,2,YUAN Yuan2,LUO Yujiang2,HUANG Luqi2*,CHEN Ping1,LI Xiaoming2(1.Wuhan Polythechnic University,Wuhan 430023,China;2.Institute of Chinese Materia Medica,China Academy of Chinese Medicinal Sciences,Beijing 100700,China)

摘要(Abstract):

目的:研究光照对黄芩悬浮细胞中黄芩苷、黄芩素的积累及相关基因表达的影响。方法:采用HPLC法检测黄芩悬浮细胞中黄芩苷、黄芩素的含量,应用半定量RT-PCR的方法对相关基因的表达进行分析。结果:光照对黄芩悬浮细胞中有效成分的积累有显著的促进作用,与黑暗条件相比,细胞生长13 d后黄芩苷含量显著增长了806.1%,黄芩素含量显著增长了42.5%,PAL基因在光照条件下的转录水平高于在黑暗条件,光照条件下UBGAT同黄芩素显著相关(r=-0.995)。结论:光诱导对黄芩悬浮细胞中有效成分的积累有显著的促进作用,光诱导刺激了与有效成分的积累相关基因(PAL,UBGAT)的表达。
Objective: To investigate the effects of light on baicalin,baicalein accumulation and related genes expression in suspension cultures of Scutellaria baicalensis.Method: The content of baicalin,baicalein in suspension cultures of S.baicalensis was determined by HPLC.The related genes expression was analyzed by semi-quantitative PCR.Result: Active ingredients in suspension cultures of S.baicalensis were promoted significantly by light,PAL gene transcription under the conditions of light was higher than that under the dark conditions,the UBGAT gene expression levels was significantly related to the baicalein(r=-0.995).Conclusion: Active ingredients and related genes(PAL,UBGAT) in suspension cultures of S.baicalensis were promoted significantly by light.

关键词(KeyWords): 黄芩;悬浮细胞;光照;半定量RT-PCR;基因表达;黄芩苷;黄芩素
Scutellaria baicalensis;suspension cells;light;semi-quantitative PCR;gene expression;baicalin;baicalein

Abstract:

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基金项目(Foundation): 北京市科技新星项目(2008B82);; 国家中医药管理局项目(06-07ZP51);; 中国中医科学院自主选题(ZZ2008062)

作者(Author): 陈顺钦;袁媛;罗毓健;黄璐琦;陈平;李晓明;
CHEN Shunqin1,2,YUAN Yuan2,LUO Yujiang2,HUANG Luqi2*,CHEN Ping1,LI Xiaoming2(1.Wuhan Polythechnic University,Wuhan 430023,China;2.Institute of Chinese Materia Medica,China Academy of Chinese Medicinal Sciences,Beijing 100700,China)

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