中国中药杂志

2010, v.35(21) 2874-2876

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UPLC测定不同产地、不同部位小叶黑柴胡中黄酮含量
Determination of flavones in different origin and parts of Bupleurum smithii var. parvifoliaum by UPLC-PDA

汤芳玲;蔡光明;袁波;张卓勇;
TANG Fangling1,2,CAI Guangming1,YUAN Bo2,ZHANG Zhuoyong3(1.China Military Institute of Chinese Materia Mdeica,302 Military Hospital,Beijing 100039,China;2.Shenyang Pharmaceutical University,Shenyang 110016,China;3.Captial Normal University,Beijing 100048,China)

摘要(Abstract):

目的:建立一种超高效液相色谱法(UPLC)检测不同产地、不同部位小叶黑柴胡中黄酮含量的方法。方法:Acquity UPLC BEHTMC18色谱柱(2.1 mm×50 mm,1.7μm),流动相为甲醇-0.1%H3PO4水梯度洗脱,流速0.4 mL.min-1,柱温30℃,检测波长为256 nm。结果:3个黄酮成分芦丁、槲皮素、异鼠李素分离良好,线性范围分别是0.106~1.06μg,r=0.9998(n=6);0.004 87~0.048 7μg,r=0.999 7(n=6);0.022 0~0.220μg,r=0.999 8(n=6)。结论:该方法测定小叶黑柴胡中黄酮类成分的含量,具有简便准确、可靠的优点。
An ultra performance liquid chromatography method has been developed for determination of flavones in different origin and different parts from Bupleurum smithii var.parvifoliaum.The separation was performed at 30 ℃ on an Acquity UPLC BEHTM C18 column eluted with methanol and 0.1% phosphoric acid solution as mobile phases in gradient elution.The detection wavelength was set at 256 nm and the flow rate was 0.4 mL·min-1.There flavones of rutin,quercetin and isorhamnetin were separated well,the linear calibration curves were obtained over the ranges of 0.106-1.06 μg for rutin(r=0.999 8,n=6),0.004 87-0.048 7 μg for quercetin(r=0.999 7,n=6),0.022 0-0.220 μg for isorhamnetin(r=0.999 8,n=6).The mean recoveries of the three compounds were 99.3%,97.8%,98.9% with RSD of 2.4%,3.0%,3.2%.The result showed that the method is convenient and feasible for determination of the flavone content in B.smithii var.parvifoliaum.

关键词(KeyWords): 小叶黑柴胡;芦丁;槲皮素;异鼠李素;超高效液相色谱法
Bupleurum smithii var.parvifoliaum;rutin;quercetin;isorhamnetin;ultra performance liquid chromatography

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基金项目(Foundation): 北京市政府专项(0853279)

作者(Author): 汤芳玲;蔡光明;袁波;张卓勇;
TANG Fangling1,2,CAI Guangming1,YUAN Bo2,ZHANG Zhuoyong3(1.China Military Institute of Chinese Materia Mdeica,302 Military Hospital,Beijing 100039,China;2.Shenyang Pharmaceutical University,Shenyang 110016,China;3.Captial Normal University,Beijing 100048,China)

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