中国中药杂志

2017, v.42(08) 1603-1608

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酒制对大黄中游离蒽醌在大鼠体内组织分布的影响
Effects of wine processed Rheum palmatum on tissue distribution of aloe-emodin,rhein and emodin in rats

吴育;彭晓清;姜晓燕;石美琴;杨水英;符映均;彭敏;蔡艳;蒋晟昰;许彦;
WU Yu;PENG Xiao-qing;JIANG Xiao-yan;SHI Mei-qin;YANG Shui-ying;FU Ying-jun;PENG Min;CAI Yan;JIANG Sheng-shi;XU Yan;Department of Pharmacy,Nantong Chinese Medicine Hospital;Medical School of Nantong University;

摘要(Abstract):

传统炮制理论指导思想"酒制升提",大黄经过炮制成为饮片酒大黄治疗上焦病症,如目赤肿痛,口舌生疮等疾病,炮制前后大黄药性的改变使得其临床应用侧重点不同,为研究中药炮制理论的科学内涵,该文同时建立测定大鼠组织中芦荟大黄素、大黄酸和大黄素的液-质联用方法,通过分别灌胃给予SD大鼠大黄生品和酒制品水煎液,采用LC-MS测定组织(心、肺、脑、肝、肾)中芦荟大黄素、大黄酸和大黄素的含量,探讨酒制对大黄中游离蒽醌成分在大鼠体内组织分布的影响。试验结果显示大黄酒制能明显改变芦荟大黄素、大黄酸和大黄素在大鼠体内的分布,其中各成分在心与肺组织中的分布增加,在肝和肾中的分布与生品组相比变化不大,而在脑中没有检测到3种成分,提示芦荟大黄素、大黄酸、大黄素不能透过血脑屏障。酒制对大黄中游离蒽醌成分在大鼠体内的药物组织分布有较大影响,这也验证了传统中医理论,酒大黄多用于治疗上焦病症,该研究结果为探讨大黄炮制机制提供了实验依据。
Under the traditional processing theory " wine processing could promote the efficacy",Rhubarb after wine processing could treat the upper energizer diseases such as red swelling,and breath sores. Processing changes the medicinal properties of rhubarb,and thus results in different focuses in clinical application. In this study,a sensitive and specific method was developed for the determination of aloe-emodin,rhein and emodin in rats tissue. Rhubarb raw materials and its wine processed decoction were given to SD rats respectively by gavage administration,and then the contents of aloe-emodin,rhein and emodin in the tissues( heart,lung,brain,liver,kidney) were determined by HPLC-MS to explore the effect of wine processing on free anthraquinones in rat tissues. Experimental results showed that wine processing can significantly change the distribution of aloe emodin,rhein and emodin in rats in vivo,and the distribution of these components was increased in heart and lung tissues. There was no significant change of distribution in the liver and the kidney as compared with raw product group,and these three ingredients were not detected in the brain,indicating that aloe-emodin,rhein,emodin can not pass through the blood brain barrier. Therefore,wine processing had greater effect on distribution of free anthraquinones in rat tissues. This also verified the theory of traditional Chinese medicine,providing experimental basis for rhubarb processing mechanism.

关键词(KeyWords): 芦荟大黄素;大黄酸;大黄素;组织分布;液-质联用
aloe-emodin;emodin;rhein;tissue distribution;HPLC-MS

Abstract:

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基金项目(Foundation): 江苏省南通市青年基金项目(WQ2016056)

作者(Author): 吴育;彭晓清;姜晓燕;石美琴;杨水英;符映均;彭敏;蔡艳;蒋晟昰;许彦;
WU Yu;PENG Xiao-qing;JIANG Xiao-yan;SHI Mei-qin;YANG Shui-ying;FU Ying-jun;PENG Min;CAI Yan;JIANG Sheng-shi;XU Yan;Department of Pharmacy,Nantong Chinese Medicine Hospital;Medical School of Nantong University;

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