中国中药杂志

2018, v.43(06) 1192-1200

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基于色谱/质谱和多元数据分析的市售迷迭香质量评价
Quality assessment of commercial rosemary based on liquid/gas chromatography and mass spectrography with multivariate statistical analysis

张继丹;郭威;唐艳;邵爱娟;杨滨;
ZHANG Ji-dan;GUO Wei;TANG Yan;SHAO Ai-juan;YANG Bin;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;State Key Laboratory Breeding Base of Dao-di Herbs;National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;School of Pharmaceutical Sciences,Shandong University of Traditional Chinese Medicine;Anhui University of Traditional Chinese Medicine;

摘要(Abstract):

该文采用GC-MS和UPLC技术分别建立了迷迭香挥发油及非挥发成分的指纹图谱,运用GC-MS和UPLC-Q-TOF-MS技术推测出63个挥发油成分和38个非挥发性成分,主要是含氧单萜、黄酮、二萜类成分等。多元数据分析结果显示,市售迷迭香样品在化学成分上存在差异,进口迷迭香挥发油属于摩洛哥/突尼斯型,国产者属于西班牙型;主成分分析和偏最小二乘判别分析结果表明,进口和国产迷迭香各自聚类;eucalyptol,(+)-α-pinene、鼠尾草酸等14个成分为差异成分,其中国产样品中(+)-α-pinene,o-cymene、鼠尾草酸等10个差异成分的量较进口样品高,eucalyptol,(+)-camphor等4个成分的量较进口样品低;另,迷迭香中重要活性成分迷迭香酸的量在各样品中离散度大,因此需对市售迷迭香的质量进行监控。
A GC-MS fingerprint of rosemary oil and an UPLC fingerprint of non-volatile compounds of rosemary were established.Sixty-three kinds of volatile compounds and thirty-eight kinds of non-volatile compounds were identified tentatively using GC-MS and UPLC-Q-TOF-MS,respectively,and most of them are flavonoids,oxygenated monoterpenes,and diterpenoids,etc. According to multivariate data analysis,chemical differences existed among commercial rosemary samples,for example,the essential oil of imported rosemary sample belongs to Morocco/Tunisian type and the one of domestic sample belongs to Spanish type. The results of principal components analysis and partial least squares discriminant analysis showed that the imported and domestic rosemary samples were classified into two groups and fourteen components including eucalyptol,( +)-α-pinene,and carnosic acid,were found as the discrimination markers. In comparison with imported rosemary,it can be found that the contents of ten markers such as( +)-α-pinene,o-cymene,and carnosic acid were higher,nonetheless,lower contents for the other markers,in those domestic rosemary samples. Moreover,rosmarinic acid,an important bioactive component in rosemary extract,extensively varied among different samples,indicating that the quality evaluation of rosemary should be concerned.

关键词(KeyWords): 迷迭香;成分鉴定;差异成分;主成分分析;偏最小二乘判别分析
rosemary;composition identification;discrimination markers;principal components analysis;partial least squares discriminant analysis

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基金项目(Foundation): 中医药行业科研专项(201407003)

作者(Author): 张继丹;郭威;唐艳;邵爱娟;杨滨;
ZHANG Ji-dan;GUO Wei;TANG Yan;SHAO Ai-juan;YANG Bin;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;State Key Laboratory Breeding Base of Dao-di Herbs;National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;School of Pharmaceutical Sciences,Shandong University of Traditional Chinese Medicine;Anhui University of Traditional Chinese Medicine;

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