中国中药杂志

2011, v.36(22) 3114-3118

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四逆散化学成分的UPLC-ESI/MS分析
Analysis of chemical composition of Sini San by ultra performance liquid chromatography-electrospray ionization-mass spectrometry

郭戎;束艳;张丽;曹雨诞;丁安伟;姚卫峰;
GUO Rong,SHU Yan,ZHANG Li,CAO Yudan,DING Anwei,YAO Weifeng(Nanjing University of Chinese Medicine,Nanjing 210046,China)

摘要(Abstract):

目的:采用液质联用技术(LC-MS)对四逆散中的化学成分进行定性分析。方法:采用ACQUITY UPLC BEH C18(2.1 mm×100 mm,1.7μm)色谱柱,以乙腈-0.1%甲酸为流动相梯度洗脱;质谱使用ESI离子源,正离子与负离子模式下采集数据。结果:通过正、负离子质谱信息及元素组成分析并结合对照品与相关文献数据对照,共鉴定出17个化合物。结论:通过超高效液相色谱分离,质谱测定相对分子质量及相关文献数据信息检索确定四逆散复方中的化学成分,为阐明四逆散的药效物质基础提供有力的证据。
Objective: To analyze and identify the chemical ingredients in Sini San by LC-MS.Method: These compounds were analyzed with an ACQUITY UPLC BEH C18(2.1 mm×100 mm,1.7 μm) by gradient elution of methyl cyanides and 0.1% formic acid.ESI source was used and data were collected in positive and negative mode.Result: Seventeen compounds of Sini San were identified by direct comparison in both positive and negative modes of Q-TOF-MS,the elemental compositions analysis,the standard substance and the data of the literature.Conclusion: It is a rapid and accurate method that the composition of compound prescription of traditional Chinese medicine can be identified in terms of the separation of ultra high performance liquid chromatography,the accurate molecurlar weights measured by MS and other information,which can trace to the source of compounds in Sinisan.

关键词(KeyWords): 四逆散;化学成分;UPLC-ESI-MS
Sini San;chemical composition;UPLC-ESI-MS

Abstract:

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基金项目(Foundation): 国家自然科学基金项目(81001599);; 教育部博士点新教师基金项目(20103237120010);; 南京中医药大学青年自然科学基金项目(09XZR14)

作者(Author): 郭戎;束艳;张丽;曹雨诞;丁安伟;姚卫峰;
GUO Rong,SHU Yan,ZHANG Li,CAO Yudan,DING Anwei,YAO Weifeng(Nanjing University of Chinese Medicine,Nanjing 210046,China)

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