中国中药杂志

2010, v.35(16) 2103-2105

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锦灯笼药材质量标准研究
Investigation on quality standard of Franchet groundcherry

程雪梅;张初航;侴桂新;王峥涛;
CHENG Xuemei1,2,ZHANG Chuhang1,3,CHOU Guixin 1,2,WANG Zhengtao1,2 (1.Shanghai Research and Development Centre for Standardization of Chinese Medicines,Shanghai 201203,China;2.MOE Key Laboratory of Standardization of Chinese Medicines of Ministry Institute of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

摘要(Abstract):

目的:建立锦灯笼药材的质量标准。方法:采用薄层鉴别,以硅胶HSG为薄层板,氯仿-丙酮-甲醇(25∶1∶1)为展开剂,薄层色谱在紫外光灯(365 nm)下检识,酸浆苦味素L荧光斑点清晰,分离效果理想。高效液相色谱方法采用VenusilXBP C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.2%磷酸(20∶80)为流动相,检测波长350 nm,流速1 mL.min-1对锦灯笼中的木犀草苷含量进行测定。结果:木犀草苷含量在0.50~249.40 mg.L-1呈良好的线性关系Y=55 313X+3.164 1,r=1.000。平均回收率分别98.79%,RSD 1.1%,日内和日间精密度RSD均分别小于2%。结论:建立的定性和定量方法可用于锦灯笼药材的质量控制。
Objective: To develop identification and assay methods of Franchet groundcherry.Method: TLC method was used to identify of physalin L in the sample using high performance silica gel G plate and a mixture of chloroform-acetone-methanol(25∶1∶1) as a developing solvent.In the chromatogram,physalin L showed a distinct fluorescence spot under UV 365 nm with good separation.In the HPLC method,luteoloside was separated on a Venusil XBP C18(4.6 mm×250 mm,5 μm) column with acetonitrile-0.2%phosphoric acid(20∶80) as the mobile phase with flow rate of 1 mL·min-1.The detection wavelength was set at 350 nm.Result: For the HPLC quantitation method,the calibration curve of luteoloside displayed ideal linearity over the range of 0.50-249.40 mg·L-1 with the regression equation of Y=55 313X+3.164 1(r=1.000).The average recovery of luteoloside was 98.79%with a RSD of 1.1%.And the intra-day and inter-day precisions were less than 2%.Conclusion: The TLC identification and HPLC determination were sensitive,reliable and repeatable and can be applied for the quality evaluation and assessment of Franchet Groundcherry Calyx.

关键词(KeyWords): 酸浆苦味素L;木犀草苷;锦灯笼;薄层色谱;反相高效液相色谱;质量标准
Physalin L;luteoloside;Franchet groundcherry;TLC;HPLC;quality standard

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基金项目(Foundation): 《中国药典》2010版一部标准研究基金项目(YD-069);; 上海科委中药现代化专项(08DZ1970103)

作者(Author): 程雪梅;张初航;侴桂新;王峥涛;
CHENG Xuemei1,2,ZHANG Chuhang1,3,CHOU Guixin 1,2,WANG Zhengtao1,2 (1.Shanghai Research and Development Centre for Standardization of Chinese Medicines,Shanghai 201203,China;2.MOE Key Laboratory of Standardization of Chinese Medicines of Ministry Institute of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

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