中国中药杂志

2016, v.41(06) 1070-1074

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野灯心草地上部分菲类成分及其抗焦虑作用研究
Phenanthrenes from aerial part of Juncus setchuensis with anxiolyticactivity

孙璐;付茜;张婵溪;王小红;王勤辉;庞欢欢;江美芳;黄建梅;
SUN Lu;FU Qian;ZHAN Chan-xi;WANG Xiao-hong;WANG Qin-hui;PANG Huan-huan;JIANG Mei-fang;HUANG Jian-mei;School of Chinese Materia Medica,Beijing University of Chinese Medicine;

摘要(Abstract):

运用正相硅胶、Sephadex LH-20、聚酰胺、制备型HPLC等多种色谱方法从野灯心草地上部分分离纯化得到10个菲类化合物和4个其他类化合物。经质谱、NMR谱图解析鉴定结构,分别为厄弗酚(1)、灯心草酚(2)、juncuenin D(3)、去氢厄弗酚(4)、去氢灯心草酚(5)、juncuenin B(6)、dehydrojuncuenin B(7)、2-甲氧基-7-羟基-1-甲基-5-乙烯基菲(8)、2-羟基-7-羧基-1-甲基-5-乙烯基-9,10-二氢菲(9)、2-羟基-7-羧基-1-甲基-5-乙烯基菲(10)、木犀草素(11),香草酸(12)、瑞香素(13)、对香豆酸(14)。其中,化合物13为首次从该属植物中分离得到,化合物5,812为首次从该植物中分离得到。采用小鼠高架十字迷宫实验评价菲类化合物6和7的抗焦虑活性,结果表明化合物6和7均具有抗焦虑作用。
Ten phenanthrenes,two organic acids,one organic acid ester and one flavonoid were isolated from the aerial part of Juncus setchuensis by various chromatographic techniques usingsilica gel,polyamide,Sephadex LH-20 as solid phases,and preparative HPLC. Their structures were identified by MS and NMR spectroscopic data as effusol( 1),juncusol( 2),juncuenin D( 3),dehydroeffusol( 4),dehydrojuncusol( 5),juncuenin B( 6),dehydrojuncuenin B( 7),2-methoxyl-7-hydroxyl-1-methyl-5-vinyl phenanthrene( 8),2-hydroxyl-7-carboxy-1-methyl-5-vinyl-9,10-dihydrophenanthrene( 9), 2-hydroxyl-7-carboxyl-1-methyl-5-vinylphenanthrene( 10),luteolin( 11),vanillic acid( 12),daphnetin( 13),p-coumaric acid( 14),respectively. Compound 13 was isolated from the genus Juncus for the first time and compounds 5,8-12 were isolated from J. setchuensis for the first time. The elevated plus-maze( EPM) was used to evaluate the anxiolytic activity of compounds 6 and 7. Compound 6 at 5 mg·kg12为首次从该植物中分离得到。采用小鼠高架十字迷宫实验评价菲类化合物6和7的抗焦虑活性,结果表明化合物6和7均具有抗焦虑作用。
Ten phenanthrenes,two organic acids,one organic acid ester and one flavonoid were isolated from the aerial part of Juncus setchuensis by various chromatographic techniques usingsilica gel,polyamide,Sephadex LH-20 as solid phases,and preparative HPLC. Their structures were identified by MS and NMR spectroscopic data as effusol( 1),juncusol( 2),juncuenin D( 3),dehydroeffusol( 4),dehydrojuncusol( 5),juncuenin B( 6),dehydrojuncuenin B( 7),2-methoxyl-7-hydroxyl-1-methyl-5-vinyl phenanthrene( 8),2-hydroxyl-7-carboxy-1-methyl-5-vinyl-9,10-dihydrophenanthrene( 9), 2-hydroxyl-7-carboxyl-1-methyl-5-vinylphenanthrene( 10),luteolin( 11),vanillic acid( 12),daphnetin( 13),p-coumaric acid( 14),respectively. Compound 13 was isolated from the genus Juncus for the first time and compounds 5,8-12 were isolated from J. setchuensis for the first time. The elevated plus-maze( EPM) was used to evaluate the anxiolytic activity of compounds 6 and 7. Compound 6 at 5 mg·kg(-1)and 10 mg·kg(-1)and 10 mg·kg(-1)showed anxiolytic activity as well as compound 7 at 10 mg·kg(-1)showed anxiolytic activity as well as compound 7 at 10 mg·kg(-1)and 20 mg·kg(-1)and 20 mg·kg(-1).

关键词(KeyWords): 野灯心草;菲类成分;高架十字迷宫;抗焦虑作用
Juncus setchuensis;phenanthrenes;the elevated plus-maze;anxiolytic

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81173541);; 北京中医药大学研究生自主课题(2014-JYBZZ-XS-089)

作者(Authors): 孙璐;付茜;张婵溪;王小红;王勤辉;庞欢欢;江美芳;黄建梅;
SUN Lu;FU Qian;ZHAN Chan-xi;WANG Xiao-hong;WANG Qin-hui;PANG Huan-huan;JIANG Mei-fang;HUANG Jian-mei;School of Chinese Materia Medica,Beijing University of Chinese Medicine;

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