中国中药杂志

2011, v.36(22) 3189-3193

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UPLC-MS/MS研究注射用复方荭草中原儿茶酸、异荭草素和野黄芩苷的大鼠组织分布
Simultaneous determination of protocatechuic acid,isoorientin and scutellarin in rat tissues after a single intravenous administration of compound Hongcao for injection by UPLC-MS/MS

黄勇;张治蓉;郑林;何峰;陈慧;王永林;
HUANG Yong,ZHANG Zhirong,ZHENG Lin,HE Feng,CHENG Hui,WANG Yonglin(School of Pharmacy,Guiyang Medical College,Guiyang 550004,China)

摘要(Abstract):

目的:建立同时测定大鼠静脉注射复方荭草后组织器官中原儿茶酸、异荭草素和野黄芩苷含量的UPLC-MS/MS分析方法,研究3种指标成分在大鼠体内的分布特征。方法:采用Acquity UPLC系统,BEH C18柱(2.1 mm×50 mm,1.7μm,美国Waters公司),流动相为乙腈-0.1%甲酸水梯度洗脱,流速为0.35 mL.min-1。采用电喷雾电离源(ESI),扫描方式为多反应离子监测(MRM);组织样本采用机械匀浆、甲醇沉淀处理供测定。结果:3种成分的线性关系良好(r>0.99),准确度、日内和日间精密度、提取回收率均符合生物样品的分析要求。大鼠注射复方荭草后原儿茶酸、异荭草素和野黄芩苷主要分布在肾、肺、心等器官中,在脑中分布最少。结论:指标成分分布研究表明,大鼠静脉给予注射用复方荭草后,体内分布具有不均衡的特征,血流充沛组织的分布相对较多;药物在体内各组织器官消除迅速,无蓄积情况;建立的方法可特异、灵敏、快速地同时测定大鼠组织中的原儿茶酸、异荭草素和野黄芩苷。
Objective: To establish an UPLC-MS/MS method for simultaneous determination protocatechuic acid,isoorientin and scutellarin in rat tissues after a single intravenous administration of Compound Hongcao for injection,and investigate the distribution character of the three compounds.Method: Three compounds were simultameously determined by UPLC with Waters Acquity UPLC BEH C18 column.The mobile phase,consisting of acetonitrile and 0.1% aqueous formic acid,was programmed as a linear gradient.The flow rate was 0.35 mL·min-1.The compounds were ionized in the electrospray ionization ion source of the mass spectrometer and detected in the multiple reaction model.The tissue samples were homogenated and the suspension were extracted with methanol for further use.Result: The relationship between the concentration and the peak areas of the three compounds were all linear(r>0.99).The precisions,accuracy,extract recoveries and stability of the analytes meet the requirements.The method has been successfully applied to tissue distribution studies of the three compounds.The present study demonstrates that the higher tissues concentration of three components were obtained in kidney,lung and heart after a single intravenous administration of ones.Conclusion: The present study demonstrates that the three compounds have unequal distribution character.It was showed that the three compounds were mainly distributed in abundant blood-supply tissues such as kidney,lung and heart.It was also found that protocatechuic acid,isoorientin and scutellarin exceretion rapidly and have no long-term accrmulation.The method was shown to be effective,convenient,and suitable for simultaneous study the distribution of the three compounds in rat.

关键词(KeyWords): 复方荭草;原儿茶酸;异荭草素;野黄芩苷;UPLC-MS/MS;组织分布
Hongcao injection;protocatechuic acid;isoorientin;scutellarin;UPLC-MS/MS;distribution

Abstract:

Keywords:

基金项目(Foundation): 国家“重大新药创制”科技重大专项(2008ZX09101-021);; 国家自然科学基金项目(30860366)

作者(Author): 黄勇;张治蓉;郑林;何峰;陈慧;王永林;
HUANG Yong,ZHANG Zhirong,ZHENG Lin,HE Feng,CHENG Hui,WANG Yonglin(School of Pharmacy,Guiyang Medical College,Guiyang 550004,China)

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