中国中药杂志

2019, v.44(05) 927-934

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姜黄植物不同发育阶段根茎中姜黄素类和萜类代谢物的积累和合成研究
Accumulation and biosynthetic of curcuminoids and terpenoids in turmeric rhizome in different development periods

孙敬茹;卜俊玲;崔光红;马莹;赵欢;毛亚平;曾雯;郭娟;黄璐琦;
SUN Jing-ru;BU Jun-ling;CUI Guang-hong;MA Ying;ZHAO Huan;MAO Ya-ping;ZENG Wen;GUO Juan;HUANG Lu-qi;State Key Laboratory Breeding Base of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;Center for Post-doctoral Research,China Academy of Chinese Medical Sciences;

摘要(Abstract):

药用植物药效活性物质的动态积累规律无论在药材生产应用还是药用成分形成的遗传机制等基础研究方面都具有重要价值,对于指导药材采收及生物合成途径解析中实验材料选择、候选基因筛选至关重要。姜黄素类代谢物和萜类代谢物是植物姜黄的药效活性成分,为了分析植物姜黄根茎中这两类代谢物的合成旺盛时期,该研究以叶丛期、根茎膨大期和干物质积累期植物姜黄的根茎为实验材料,分析了3个发育时期的代谢变化和转录变化。结果表明,叶丛期萜类代谢物含量较高,多个与萜类生物合成相关的关键催化酶基因表达最高,干物质积累期姜黄素类代谢物含量较高,与姜黄素类代谢物合成的关键酶基因表达最高。因而,叶丛期萜类代谢物合成最旺盛,干物质积累期姜黄素类代谢物合成水平最高。该研究通过比较转录组和代谢组深入分析植物姜黄根茎姜黄素类和萜类代谢合成规律,为药材生产应用提供参考,为这些代谢物的生物合成提供研究基础。
The dynamic accumulation rule of active substances in medicinal plants is of great value not only for medicinal material production and application,but also for the genetic mechanism study on the formation of medicinal ingredients,especially vital to guide medicinal material collection as well as experiment material selection and candidate gene screening in the analysis of biosynthesis pathway. This study investigated the accumulation of curcumins and terpenoids,and the biosynthesis of these metabolites,which are the active metabolites in Curcuma longa,a commonly used traditional Chinese medicine. Rhizoma of C. longa from leaf growing period,rhizome swelling period and dry matter accumulating period were used as experimental materials,to analyze the changes of metabolites and biosynthesis in the three periods by comparative transcriptome and metabolomes analysis.The results indicated that terpenoids accumulation and biosynthesis mainly occurred in leaf growing period,while curcumin accumulation and biosynthesis mainly occurred in dry matter accumulating period. Therefore,we suggested that turmeric rhizomes in leaf growth period were suitable for terpenoids biosynthetic pathway characterization,and rhizome in accumulation of dry matter period was suitable for curcuminoid biosynthesis pathway characterization. This study provides references for medicinal materialproduction and application,as well as biopathway analysis of active compounds for C. longa.

关键词(KeyWords): 姜黄;发育时期;姜黄素类代谢物;萜类代谢物;基因表达
turmeric;development period;curcuminoids;terpenenoids;gene expression

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81603244);; 中国博士后科学基金项目(2015M571252);; 中央本级重大增减支项目(2060302)

作者(Author): 孙敬茹;卜俊玲;崔光红;马莹;赵欢;毛亚平;曾雯;郭娟;黄璐琦;
SUN Jing-ru;BU Jun-ling;CUI Guang-hong;MA Ying;ZHAO Huan;MAO Ya-ping;ZENG Wen;GUO Juan;HUANG Lu-qi;State Key Laboratory Breeding Base of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;Center for Post-doctoral Research,China Academy of Chinese Medical Sciences;

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